MiR-608 rs4919510 is associated with prognosis of hepatocellular carcinoma
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Single nucleotide polymorphisms (SNPs) within microRNAs (miRNAs) are considered potential markers for risk and prognosis of various cancers. In the current study, we aimed to determine whether miR-608 rs4919510 affected hepatocellular carcinoma (HCC) prognosis. We genotyped rs4919510 using DNA from blood samples of 362 HCC patients receiving surgical resection of HCC tumor. Associations between rs4919510 and overall survival (OS) and demographic characteristics and clinical features were estimated using the Cox proportional hazards model. Results showed that HCC patients who carried the rs4919510 CC genotype had a significantly longer OS compared to those who carried the GG genotype (P = 0.013, hazard ratio [HR] = 0.600, 95 % confidence interval [CI] 0.402–0.897) and the CG + GG genotype (P = 0.033, HR = 0.681, 95 % CI 0.479–0.970) in univariate analysis. Similar results were obtained in multivariate analysis. Further stratification analysis indicated that rs4919510 was significantly associated with OS in patients who were satisfied with one of the following criteria: male gender, HbsAg-positive, α-fetoprotein (AFP)-positive, tumor size >5 cm, cirrhosis, solitary tumor, I + II pTNM stage, or no tumor capsule. Finally, a significantly higher frequency of rs4919510 CC genotype was observed in patients with cirrhosis (22.9 %, 55/240) than those without cirrhosis (14.0 %, 17/121) (P = 0.047). In conclusion, our results illustrated the potential role of miR-608 rs4919510 as a prognostic marker for HCC patients undergoing surgical resection of the tumor.
KeywordsHepatocellular carcinoma Survival miRNA Genetic polymorphism
We thank all the patients who agreed to participate in this study. The study is supported by the National Natural Science Foundation of China (31100895, and 81472618 [to D.-K.J.]), Outstanding Young Scholar Project of Fudan University (to D.-K.J.), as well as an intramural research grant for new young teachers from Fudan University (to D.-K.J.), an intramural research grant for promotion of the scientific research ability of young teachers from Fudan University (to D.-K.J.), an intramural research grant from Huashan Hospital, Fudan University (to J.X.), an intramural research grant from Fudan-VARI Center for Genetic Epidemiology, Fudan University (to J.X.)., the Research Fund of the State Key Laboratory of Genetic Engineering, Fudan University (to L.Y. and J.X.), and the Ellrodt-Schweighauser Family Chair of Cancer Genomic Research of NorthShore University HealthSystem (J. X.).
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Conflicts of interest
- 15.Lin M, Gu J, Eng C, Ellis LM, Hildebrandt MA, Lin J, et al. Genetic polymorphisms in microRNA-related genes as predictors of clinical outcomes in colorectal adenocarcinoma patients. Clin Cancer Res: Off J Am Assoc Cancer Res. 2012;18(14):3982–91. doi: 10.1158/1078-0432.CCR-11-2951.CrossRefGoogle Scholar
- 26.Jazdzewski K, Murray EL, Franssila K, Jarzab B, Schoenberg DR, de la Chapelle A. Common SNP in pre-miR-146a decreases mature miR expression and predisposes to papillary thyroid carcinoma. Proc Natl Acad Sci U S A. 2008;105(20):7269–74. doi: 10.1073/pnas.0802682105.CrossRefPubMedPubMedCentralGoogle Scholar
- 34.Kang JG, Majerciak V, Uldrick TS, Wang X, Kruhlak M, Yarchoan R, et al. Kaposi’s sarcoma-associated herpesviral IL-6 and human IL-6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation. J Pathol. 2011;225(3):378–89. doi: 10.1002/path.2962.CrossRefPubMedPubMedCentralGoogle Scholar