Tumor Biology

, Volume 37, Issue 8, pp 10187–10191 | Cite as

Effect of Shenfu injection on immune function of mice bearing Lewis lung sarcoma with chemotherapy

Original Article


Chemotherapy damages immune system and has a lot of side effects. In this study, we investigate the function of Shenfu injection to reduce these unfavorable effects induced by chemotherapy on mice bearing Lewis lung sarcoma. Mice inoculated with Lewis lung sarcoma cells were divided into five groups: Lewis lung sarcoma control group, cyclophosphamide (CTX) group, and Shenfu injection (high, moderate, and low dose) + CTX group. After a 14-day treatment, the counting of peripheral blood cells, CD3+, CD4+, and CD8+ T lymphocytes were done, immunoglobulin (Ig) was measured, coefficients of spleen and thymus were calculated, and spleen T cell proliferation was evaluated in vitro. The CD4+/CD8+ and CD3+ T cells in high- and moderate-dose Shenfu groups were more than the CTX group (p < 0.05); spleen T cell proliferation of mice in high-dose Shenfu + CTX group is more prominent than the CTX group (p < 0.05); coefficients of spleen and thymus, WBC, and platelet (PLT) counting of mice in the CTX group were lower than control and high and moderate dose Shenfu + CTX groups. The level of serous IgG and IgM of all test groups shows no significant difference. Shenfu injection can improve cellular immune function and reduce myolosuppression of mice delivered with chemotherapy.


Shenfu injection Mice Lewis lung sarcoma Chemotherapy Immune function 


Compliance with ethical standards

All experiments were carried out in accordance with the institutional guidelines, and the study protocol was approved by the Institutional Animal Care and Use Committee (IACUC) at the Sichuan Cancer Hospital.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Yukun Luo
    • 1
  • Kangning Wang
    • 2
  • Qiang Li
    • 2
  • Jinyi Lang
    • 1
  1. 1.Department of Radiation OncologySichuan Cancer Hospital and InstituteChengduChina
  2. 2.Department of Thoracic SurgerySichuan Cancer Hospital and InstituteChengduChina

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