Tumor Biology

, Volume 37, Issue 8, pp 10187–10191 | Cite as

Effect of Shenfu injection on immune function of mice bearing Lewis lung sarcoma with chemotherapy

Original Article
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Abstract

Chemotherapy damages immune system and has a lot of side effects. In this study, we investigate the function of Shenfu injection to reduce these unfavorable effects induced by chemotherapy on mice bearing Lewis lung sarcoma. Mice inoculated with Lewis lung sarcoma cells were divided into five groups: Lewis lung sarcoma control group, cyclophosphamide (CTX) group, and Shenfu injection (high, moderate, and low dose) + CTX group. After a 14-day treatment, the counting of peripheral blood cells, CD3+, CD4+, and CD8+ T lymphocytes were done, immunoglobulin (Ig) was measured, coefficients of spleen and thymus were calculated, and spleen T cell proliferation was evaluated in vitro. The CD4+/CD8+ and CD3+ T cells in high- and moderate-dose Shenfu groups were more than the CTX group (p < 0.05); spleen T cell proliferation of mice in high-dose Shenfu + CTX group is more prominent than the CTX group (p < 0.05); coefficients of spleen and thymus, WBC, and platelet (PLT) counting of mice in the CTX group were lower than control and high and moderate dose Shenfu + CTX groups. The level of serous IgG and IgM of all test groups shows no significant difference. Shenfu injection can improve cellular immune function and reduce myolosuppression of mice delivered with chemotherapy.

Keywords

Shenfu injection Mice Lewis lung sarcoma Chemotherapy Immune function 

Notes

Compliance with ethical standards

All experiments were carried out in accordance with the institutional guidelines, and the study protocol was approved by the Institutional Animal Care and Use Committee (IACUC) at the Sichuan Cancer Hospital.

Conflicts of interest

None.

References

  1. 1.
    Fu YK, Xie YM. Investigation of clinical practice and side effects of Shenfu injection. Zhongguo Zhong yao za zhi. 2012;37(18):2796–9.PubMedGoogle Scholar
  2. 2.
    Gu W, Li C, Yin W, Hou X, Zhang D. Effects of Shen-Fu injection on the expression of t-cell-specific transcription factors T-bet/Gata-3 in porcine postresuscitation lung injury. Evid Based Complement Alternat Med. 2013;2013:464650.PubMedPubMedCentralGoogle Scholar
  3. 3.
    Gu Y, Xu H, Zhao M. Clinical study on effect of Shenfu injection treating cancer-related fatigue of patients with advanced carcinoma. Zhongguo Zhong yao za zhi. 2010;35(7):915–8.PubMedGoogle Scholar
  4. 4.
    Hu J, Fu ZY, Xie YM, Wang J, Wang WW, Liao X. Systematic review of shenfu injection for septic shock. Zhongguo Zhong yao za zhi. 2013;38(18):3209–14.PubMedGoogle Scholar
  5. 5.
    Ke DZ, Chen QW, Li CL, Li GQ. Cytokines mechanism of shenfu injection in treatment of cardiogenic shock in canine. Zhongguo Zhong yao za zhi. 2007;32(21):2273–7.PubMedGoogle Scholar
  6. 6.
    Li H, Wang YG, Ma ZC, Zhou SS, Liang QD, Xiao CR, et al. Effect of shenfu injection on CYP450s of rat liver. Yao Xue Xue Bao. 2013;48(5):728–33.PubMedGoogle Scholar
  7. 7.
    Long SQ, Liao GY, He WF, Wang B, Deng H, Zhang HB, et al. Influence of Shenfu injection on the quality of life of lung cancer patients receiving chemotherapy. Nan Fang Yi Ke Da Xue Xue Bao. 2011;31(12):2090–2.PubMedGoogle Scholar
  8. 8.
    Lu LG, Zhang X, Chen ZQ, Bai ZG, Wu QL, Wang ZH, et al. Shenfu injection induces the apoptosis of prostate cancer PC-3 cells. Zhonghua Nan Ke Xue. 2014;20(6):539–43.PubMedGoogle Scholar
  9. 9.
    Luo ZQ, Feng DD, Yue SJ. Effect of Shenfu injection on the synthesis of pulmonary surfactant in cultured lung explants. Hunan Yi Ke Da Xue Xue Bao. 2003;28(2):137–40.PubMedGoogle Scholar
  10. 10.
    Ma M. Clinical observation on effect of combined therapy of pamidronati sodium and Shenfu injection in treating multiple myeloma caused ostealgia. Zhongguo Zhong xi yi jie he za zhi. 2004;24(1):67–8.PubMedGoogle Scholar
  11. 11.
    Tian DL, Li K, Jiang JJ, Zhu YN. Risk control system construction of Shenfu injection. Zhongguo Zhong yao za zhi. 2012;37(18):2814–7.PubMedGoogle Scholar
  12. 12.
    Tian X, Gou DM, Cai HJ. Effect of Shenfu injection on the erythrocyte immune function of patients undergoing cardiopulmonary bypass. Zhongguo Zhong xi yi jie he za zhi. 2011;31(11):1471–3.PubMedGoogle Scholar
  13. 13.
    Wang LY, Wei YF, Du HL. Effect of shenfu injection on recovery of intestinal function, cellular immunity, serum interleukin-2 and tumor necrosis factor-alpha in children with intestinal obstruction after operation. Zhongguo Zhong xi yi jie he za zhi. 2003;23(10):744–6.PubMedGoogle Scholar
  14. 14.
    Wang SY, Wei YF, Du HL, Ren LL, Li SH. Clinical efficacy and T-lymphocyte subset, serum interferon-gamma (IFN-gamma), tumor necrosis factor-alpha (TNF-alpha), interleukin-2(IL-2) levels on treatment of chronic aplastic anemia patients by Shenfu injection combined with stanozol and cyclosporin A. Zhongguo Zhong yao za zhi. 2005;30(5):383–5.PubMedGoogle Scholar
  15. 15.
    Wu WY, Long SQ, Chai XS. Effect of Shenfu injection for attenuating the toxicity of cisplatin-based regimens in treating non-small cell lung cancer. Zhongguo Zhong xi yi jie he za. 2009;29(1):19–22.Google Scholar
  16. 16.
    Wu WY, Long SQ, Zhang HB, Chai XS, Deng H, Xue XG, et al. Improvement of quality of life with Shenfu injection in non small cell lung cancer patients treated with gemcitabine plus cisplatin regimen. Chin J Integr Med. 2006;12(1):50–4.CrossRefPubMedGoogle Scholar
  17. 17.
    Yang F, Zheng Y, Li D, Deng W. Effect of Shenfu injection on microcirculation. Sheng wu yi xue gong cheng xue za zhi. 2003;20(1):91–4. 100.PubMedGoogle Scholar
  18. 18.
    Yang J, Zhao RQ, Xie YM, Yang H, Li L, Zhuang Y. Clinical outcomes research of use of Shenfu injection based on hospital information system. Zhongguo Zhong yao za zhi. 2012;37(18):2730–4.PubMedGoogle Scholar
  19. 19.
    Zeng ZG, Gong HH, Li Y, Nie ZY, Jie KM, Zhan YA, et al. Effect of Shenfu injection on expression of lipopolysaccharide-induced microRNA-146a in alveolar macrophages. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2012;24(3):166–9.PubMedGoogle Scholar
  20. 20.
    Zhang Q, Li CS, Wang S, Gu W. Effects of Chinese medicine Shen-Fu injection on the expression of inflammatory cytokines and complements during post-resuscitation immune dysfunction in a porcine model. Chin J Integr Med. 2014. doi: 10.1007/s11655-014-1857-8.Google Scholar
  21. 21.
    Zhou L, Li H, Zhang Y, Han S, Xu H. Development of genus-specific primers for better understanding the diversity and population structure of Sphingomonas in soils. J Basic Microbiol. 2014;54(8):880–8.CrossRefPubMedGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Yukun Luo
    • 1
  • Kangning Wang
    • 2
  • Qiang Li
    • 2
  • Jinyi Lang
    • 1
  1. 1.Department of Radiation OncologySichuan Cancer Hospital and InstituteChengduChina
  2. 2.Department of Thoracic SurgerySichuan Cancer Hospital and InstituteChengduChina

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