Tumor Biology

, Volume 37, Issue 8, pp 10469–10478 | Cite as

Roles of CDKN1A gene polymorphisms (rs1801270 and rs1059234) in the development of cervical neoplasia

  • Sandra Liliana Vargas-Torres
  • Elyzabeth Avvad Portari
  • Amanda Lima Silva
  • Evandro Mendes Klumb
  • Heloísa Carneiro da Rocha Guillobel
  • Maria José de Camargo
  • Cíntia Barros Santos-Rebouças
  • Fábio Bastos Russomano
  • Jacyara Maria Brito Macedo
Original Article

Abstract

The CDKN1A gene product is a p53 downstream effector, which participates in cell differentiation, development process, repair, apoptosis, senescence, migration, and tumorigenesis. The objective of our study was investigated the importance of two polymorphisms in the CDKN1A gene, rs1801270 (31C>A) and rs1059234 (70C>T), for the development of cervical lesions in a Southeastern Brazilian population (283 cases, stratified by lesion severity, and 189 controls). CDKN1A genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and/or DNA sequencing. CDKN1A 31A allele presents a genetic pattern of protection for the development of high-grade cervical lesions (CC vs CA genotype: OR = 0.60; 95 % CI = 0.38–0.95; p = 0.029; CA+AA vs CC genotype: OR = 0.60; 95 % CI = 0.39–0.93; p = 0.021). Allele distributions of the CDKN1A 70C>T polymorphism were also different between the two study groups, with the CDKN1A 70T allele being less prevalent among cases. Moreover, the double heterozygote genotype combination 31CA-70CT decreases the chance of developing high-grade squamous intraepithelial lesion (HSIL) and cancer (OR = 0.55; 95 % CI = 0.32–0.93; p = 0.034) by 50 %, representing a protective factor against the development of more severe cervical lesions.

Keywords

CDKN1A Cervical neoplasia rs1801270 rs1059234 p21 Polymorphism 

Notes

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Sandra Liliana Vargas-Torres
    • 1
  • Elyzabeth Avvad Portari
    • 2
    • 3
  • Amanda Lima Silva
    • 1
  • Evandro Mendes Klumb
    • 4
  • Heloísa Carneiro da Rocha Guillobel
    • 5
  • Maria José de Camargo
    • 6
  • Cíntia Barros Santos-Rebouças
    • 7
  • Fábio Bastos Russomano
    • 6
  • Jacyara Maria Brito Macedo
    • 1
  1. 1.Department of Biochemistry, Biology InstituteState University of Rio de Janeiro - UERJRio de JaneiroBrazil
  2. 2.Department of PathologyState University of Rio de Janeiro - UERJRio de JaneiroBrazil
  3. 3.Department of PathologyFernandes Figueira Institute - FIOCRUZRio de JaneiroBrazil
  4. 4.Department of RheumatologyState University of Rio de Janeiro - UERJRio de JaneiroBrazil
  5. 5.Department of Biophysics and BiometryState University of Rio de Janeiro - UERJRio de JaneiroBrazil
  6. 6.Department of GynecologyFernandes Figueira Institute - FIOCRUZRio de JaneiroBrazil
  7. 7.Department of GeneticsState University of Rio de Janeiro - UERJRio de JaneiroBrazil

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