Tumor Biology

, Volume 37, Issue 7, pp 9473–9481 | Cite as

Residual expression of SMYD2 and SMYD3 is associated with the acquisition of complex karyotype in chronic lymphocytic leukemia

  • Wilson Oliveira-Santos
  • Doralina Amaral Rabello
  • Antônio Roberto Lucena-Araujo
  • Fábio Morato de Oliveira
  • Eduardo Magalhaes Rego
  • Fábio Pittella Silva
  • Felipe Saldanha-Araujo
Original Article


SET and MYND domain containing 2 (SMYD2) and the SET and MYND domain containing 3 (SMYD3) are the most studied and well-characterized members of SMYD family. It has been demonstrated that their altered expression is associated with the progression of several solid tumors. Nevertheless, whether these methyltransferases exert any impact in chronic lymphocytic leukemia (CLL) remains unknown. Here, we investigated the gene expression profile of SMYD2 and SMYD3 in 59 samples of CLL and 10 normal B cells. The obtained results were associated with white blood cells (WBC) and platelet counts, ZAP-70 protein expression, and cytogenetic analysis. We found that SMYD2 and SMYD3 are overexpressed in CLL patients and, interestingly, patients with residual expression of both genes presented a high WBC count and complex karyotype. Furthermore, a strong correlation between SMYD2 and SMYD3 gene expression was unveiled. Our data demonstrate the association of a residual expression of SMYD2 and SMYD3 with CLL progression indicators and suggests both genes are regulated by a common transcriptional control in this type of cancer. These results may provide the basis for the development of new therapeutic strategies to prevent CLL progression.


Chronic lymphocytic leukemia Cytogenetic Complex karyotype SMYD ZAP-70 



The present study was funded by CNPq (National Council of Technological and Scientific Development), CAPES (Coordination for the Improvement of Higher Education Personnel), and FAPDF (Federal District Research Foundation).

Authors’ contribution

WOS and DAR were both responsible for conception and design, collection and/or assembly of data, data analysis and interpretation, and manuscript writing. ARLA was both responsible for conception and design, provision of study material or patients, collection and/or assembly of data, and manuscript writing. FMO was both responsible for conception and design, provision of study material or patients, and collection and/or assembly of data. EMR was both responsible for provision of study material or patients and collection and/or assembly of data. FPS was both responsible for conception and design, provision of study material or patients, data analysis, and manuscript writing. FSA was both responsible for conception and design, provision of study material or patients, data analysis, and manuscript writing. All authors read and approved the final manuscript.

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Wilson Oliveira-Santos
    • 1
  • Doralina Amaral Rabello
    • 1
  • Antônio Roberto Lucena-Araujo
    • 2
  • Fábio Morato de Oliveira
    • 2
  • Eduardo Magalhaes Rego
    • 2
  • Fábio Pittella Silva
    • 1
  • Felipe Saldanha-Araujo
    • 1
  1. 1.Laboratory of Molecular Pathology of Cancer, Faculty of Health SciencesUniversity of BrasíliaBrasíliaBrazil
  2. 2.Department of Internal Medicine, Medical School of Ribeirão PretoUniversity of São PauloRibeirão PretoBrazil

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