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Tumor Biology

, Volume 37, Issue 7, pp 9527–9534 | Cite as

MicroRNAs modulating angiogenesis: miR-129-1 and miR-133 act as angio-miR in HUVECs

  • Mina Soufi-zomorrod
  • Abbas Hajifathali
  • Fatemeh Kouhkan
  • Mahshid Mehdizadeh
  • Seyed Mohammad Ali Hosseini Rad
  • Masoud Soleimani
Original Article

Abstract

The sprouting of new blood vessels by angiogenesis is critical in vascular development and homeostasis. Aberrant angiogenesis leads to enormous pathological conditions such as ischemia and cancer. MicroRNAs (also known as miRNAs or miRs) play key roles in regulation of a range of cellular processes by posttranscriptional suppression of their target genes. Recently, new studies have indicated that miRNAs are involved in certain angiogenic settings and signaling pathways use these non-coding RNAs to promote or suppress angiogenic processes. Herein, VEGFR2 and FGFR1 were identified as miR-129-1 and miR-133 targets using bioinformatic algorithms, respectively. Afterwards, using luciferase reporter assay and gene expression analysis at both mRNA and protein levels, VEGFR2 and FGFR1 were validated as miR-129-1 and miR-133 targets. In addition, we showed that miR-129-1 and miR-133 suppress angiogenesis properties such as proliferation rate, cell viability, and migration activity of human umbilical vein endothelial cells (HUVEC) in vitro. We conclude that these miRNAs can suppress key factors of angiogenesis by directly targeting them. These results have important therapeutic implications for a variety of diseases involving deregulation of angiogenesis, including cancer.

Keywords

MiR-129-1 MiR-133 Angiogenesis VEGFR2 FGFR1 HUVEC cells 

Notes

Acknowledgments

This project has been funded by Tarbiat Modares University and Stem Cell Technology Research Center, Tehran, Iran. The authors also thank Arash Veshkini for assistance in preparing the Figures.

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Mina Soufi-zomorrod
    • 1
  • Abbas Hajifathali
    • 2
  • Fatemeh Kouhkan
    • 3
  • Mahshid Mehdizadeh
    • 4
  • Seyed Mohammad Ali Hosseini Rad
    • 3
  • Masoud Soleimani
    • 1
  1. 1.Department of Hematology, School of Medical SciencesTerbiat Modares UniversityTehranIran
  2. 2.Department of Medical GeneticsShahid Beheshti University of Medical SciencesTehranIran
  3. 3.Stem Cell Technology Research CenterTehranIran
  4. 4.Shahid Beheshti University of Medical ScienceTehranIran

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