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Tumor Biology

, Volume 37, Issue 8, pp 10161–10175 | Cite as

Cyclin Y regulates the proliferation, migration, and invasion of ovarian cancer cells via Wnt signaling pathway

  • Haiyuan Liu
  • Honghui Shi
  • Qingbo Fan
  • Xiangxiu Sun
Original Article

Abstract

This study is designated to investigate the roles of cyclin Y (CCNY) and Wnt signaling pathway in regulating ovarian cancer (OC) cell proliferation, migration, and invasion. Quantitative real-time PCR (qRT-PCR), Western blot, MTT assay, cell scratch, and transwell test were used in our study, and transplanted tumor model was constructed on nude mice. C-Myc, cyclin D1, PFTK1, ki67, OGT, and β-catenin protein expressions in tumor tissues were detected. CCNY was significantly upregulated in OC cell lines and tissues (both P < 0.05); significant association was observed between CCNY expression and clinicopathological stage, lymph node metastasis (LNM) (P < 0.05); and the CCNY expression in stages III to IV was higher than that in stages I to II, and patients with LNM had higher CCNY expression when compared with those in patients without LNM (P < 0.05); expressions of c-Myc, cyclin D, PFTK1, ki67, and OGT were upregulated in OC tissues compared with ovarian benign tissues, suggesting that these expressions were significantly different between the two groups (P < 0.05); CCNY significantly exacerbated proliferation, migration, and invasion of A2780 cells; c-Myc and cyclin D1 protein expressions increased as the expression of CCNY increased (P < 0.001); β-catenin expressions in A2780 cells with over-expression of CCNY were significantly increased in the nucleus, but significantly decreased in the cytoplasm (both P < 0.05); high expressions of CCNY exacerbated the proliferation of A2780 cells in nude mice and significantly increased c-Myc, cyclin D1, PFTK1, ki67, and OGT protein expressions in tumor tissues which were transplanted into nude mice (P < 0.01). CCNY might exacerbate the proliferation, migration, and invasion of OC cells via activating the Wnt signaling pathway. Thus, this study provides a theoretical foundation for the development of therapeutic drugs that are able to cure OC by targeting CCNY.

Keywords

Ovarian cancer Proliferation Migration Invasion CCNY Wnt signaling pathway Cyclin Y β-catenin 

Notes

Acknowledgments

We would like to express our sincere appreciation to the reviewers for their constructive comments on this article.

Compliance with ethical standards

The experimental protocol was approved by the Ethics Committee of Animal Experiment Center of Peking Union Medical College Hospital and complied with the standards with respect to animal experiment published by the US National Institutes of Health in 1996.

Conflicts of interest

None

Ethics approval and informed consent

This study was approved by the ethics committee of Peking Union Medical College Hospital, and all patients signed the informed consent. The implementation of this study complied with the standards set by the Declaration of Helsinki.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Haiyuan Liu
    • 1
  • Honghui Shi
    • 1
  • Qingbo Fan
    • 1
  • Xiangxiu Sun
    • 2
  1. 1.Department of Obstetrics and GynecologyPeking Union Medical College HospitalBeijingChina
  2. 2.Department of Obstetrics and GynecologyLinyi People’s HospitalLinyiChina

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