Tumor Biology

, Volume 37, Issue 7, pp 9433–9440 | Cite as

HPV 16 E7 inhibits OSCC cell proliferation, invasion, and metastasis by upregulating the expression of miR-20a

  • Jun Hu
  • Weili Ge
  • Junfeng Xu
Original Article


The aim of this research was to study how HPV-16 E7 affects the proliferation, invasion, and metastasis of oral squamous cell carcinoma (OSCC) cells by upregulating the expression of miR-20a. A total of 60 OSCC patients were included in this study. SiRNA-198 was used to inhibit HPV-16 E7, and the constructed plasmid of HPV-16 E7 was transfected into Cal27 cells. Then, HPV-16 E7 protein was detected by Western blot and RT-PCR was performed to measure miR-20a expression in OSCC cells. Either HPV-16 E7 or the combination of HPV-16 E7 and miR-20a inhibitors was transfected into Cal27 cells separately. And then, the effect of miR-20a on OSCC cells proliferation was evaluated by CCK-8. Moreover, transwell assay and wound healing assay were used to assess the impact of miR-20a on OSCC cell invasion migration. MiR-20a was significantly higher in OSCC tissues compared with para-carcinoma tissues. RT-PCR results indicated that miR-20a was downregulated after silencing HPV-16 E7. By contrast, miR-20a was upregulated after the overexpression of HPV-16 E7. Upregulation of miR-20a by transfected plasmid HPV-16 E7 can significantly inhibit Cal27 cell proliferation, invasion, and migration. The expression of MiR-20a upregulated by HPV-16 E7 inhibits the proliferation, invasion, and migration of OSCC cells.


HPV-16 E7 MiR-20a OSCC Proliferation Invasion Migration 



The authors wish to express their appreciation to the reviewers for their critical comments.

Compliance with ethical standard

This research on human subjects was approved by the Ethics Committee of Hospital of Stomatology Affiliated to the Zhejiang University. Written informed consent was obtained from each participant. All experiments were performed in accordance with relevant approved guidelines and regulations of Hospital of Stomatology Affiliated to Zhejiang University.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  1. 1.Department of ProsthodonticsHospital of Stomatology Affiliated to Zhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Department of Maxillofacial SurgeryHospital of Stomatology Affiliated to Zhejiang UniversityHangzhouPeople’s Republic of China
  3. 3.Department of StomatologyTongde Hospital of Zhejiang ProvinceHangzhouPeople’s Republic of China

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