Tumor Biology

, Volume 37, Issue 7, pp 9181–9187 | Cite as

MicroRNA-503 represses epithelial–mesenchymal transition and inhibits metastasis of osteosarcoma by targeting c-myb

  • Xinzhen Guo
  • Jie Zhang
  • Jianfeng Pang
  • Sheng He
  • Guojun Li
  • Yang Chong
  • Chao Li
  • Zhijian Jiao
  • Shiqian Zhang
  • Ming Shao
Original Article

Abstract

Deregulated expression of miRNAs contributes to the development of osteosarcoma. Our previous study has showed that miR-503 was downregulated in osteosarcoma tissues. However, the mechanism of the miR-503 in osteosarcoma development still remains largely undefined. In our study, we found that miR-503 overexpression suppressed cell invasion and migration and inhibited epithelial-to-mesenchymal transition (EMT) of MG-63. Furthermore, we identified that c-myb, an oncogene, was a direct target of miR-503. Moreover, overexpression of c-myb could rescue miR-503-suppressed invasion and EMT. The expression of c-myb was upregulated in osteosarcoma cell lines. Therefore, we conclude that high miR-503 expression suppressed osteosarcoma cell mobility and EMT through targeting c-myb, and this may serve as a therapeutic target.

Keywords

Osteosarcoma MicroRNA miR-503 c-myb EMT 

Notes

Acknowledgments

This work was supported by grants from the National Natural Science Foundation of Heilongjiang Province (Grant Numbers: H201308).

Compliance with ethical standards

Conflicts of interest

None

Supplementary material

13277_2016_4797_MOESM1_ESM.docx (14 kb)
Table S1 (DOCX 13 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Xinzhen Guo
    • 1
  • Jie Zhang
    • 1
  • Jianfeng Pang
    • 2
  • Sheng He
    • 3
  • Guojun Li
    • 1
  • Yang Chong
    • 1
  • Chao Li
    • 1
  • Zhijian Jiao
    • 1
  • Shiqian Zhang
    • 1
  • Ming Shao
    • 1
  1. 1.Department of Orthopedic SurgeryThe First Affiliated Hospital of Harbin Medical UniversityHarbinChina
  2. 2.Ming Shui County People’s HospitalSuihuaChina
  3. 3.Administration Center Hospital of NongkenBei’anChina

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