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Tumor Biology

, Volume 37, Issue 7, pp 9849–9854 | Cite as

The tumor promoting roles of HSP60 and HIF2α in gastric cancer cells

Original Article

Abstract

The roles of HSP60 and HIF2α in diagnosis, prognosis, and prevention and treatment of various human cancers have been detected. However, the combined roles of HSP60 and HIF2α on the prognosis of patients with gastric cancer remain unclear. In this work, we confirmed that the levels of HSP60 and HIF2α messenger RNA (mRNA) and protein were higher in gastric cancer tissues than that in matched normal tissues by using real-time PCR and Western blot. Furthermore, we confirmed that inhibition of HSP60 or HIF2α could induce apoptosis and inhibit cell mobility. Co-immunoprecipitation (co-IP) was performed to determine the interaction between HSP60 and HIF2α. Lastly, we confirmed that knockdown of HSP60 or HIF2α induced apoptosis in gastric cancer cells is negatively related to the MEK/ERK signaling in vitro. In summary, HSP60 or HIF2α protein expression may be a predictive marker for the prognosis of the patients with gastric cancer. Targeting HSP60 and HIF2α could be a future strategy to improve survival of gastric patients with poor prognosis.

Keywords

HIF2α HSP60 Apoptosis Mobility Survival rate 

Notes

Acknowledgments

We thank Dr. Pu Xia for technical assistance.

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Wei-Wei Tong
    • 1
  • Guang-Hui Tong
    • 1
  • Hong Kong
    • 1
  • Yong Liu
    • 1
    • 1
  1. 1.Department of Laboratory MedicineShengjing Hospital of China Medical UniversityShenyangPeople’s Republic of China

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