Tumor Biology

, Volume 37, Issue 7, pp 9189–9196 | Cite as

Caveolin-1 affects tumor drug resistance in esophageal squamous cell carcinoma by regulating expressions of P-gp and MRP1

  • Song Zhang
  • Wenbo Cao
  • Mingjin Yue
  • Naigang Zheng
  • Tao Hu
  • Shengli Yang
  • Ziming Dong
  • Shixin Lu
  • Saijun Mo
Original Article

Abstract

Esophageal squamous cell carcinoma (ESCC) is the most common cancer in China, and multidrug resistance (MDR) remains one of the biggest problems in ESCC chemotherapy. In this study, we aimed to investigate the mechanism of Caveolin-1, an integral membrane protein, on regulating ESCC MDR. First, immunohistochemistry was used to check the protein expression of Caveolin-1, MDR-related protein of P-glycoprotein (P-gp), and multidrug resistance protein 1 (MRP1) in 84 pathologically characterized ESCC tissues, matched adjacent tumor, and adjacent normal-looking tissues. The results showed that Caveolin-1 expression level was elevated in ESCC tissues than that of matched adjacent tumor and adjacent normal-looking tissues (P < 0.05), and the expression of Caveolin-1 has close correlation with P-gp and MRP1 during tumor genesis of ESCC (P = 0.034, P = 0.009, respectively). Then, Caveolin-1 overexpression and knockdown were used to investigate its effect on expressions of P-gp and MRP1 in ESCC cell line Ec9706. The messenger RNA (mRNA) and protein expression levels of P-gp and MRP1 were checked by real-time quantitative reverse transcription-PCR (qRT-PCR) and Western blot (WB). The results showed that Caveolin-1 overexpression significantly promotes the mRNA and protein expression of MRP1 (P < 0.05), while almost has no effect on the mRNA and protein expression of P-gp (P > 0.05); Cavoelin-1 knockdown inhibits the mRNA and protein expressions of both P-gp and MRP1 (P < 0.05). The similar result was found in another ESCC cell line Eca109. So, it is concluded that Caveolin-1 affects ESCC MDR by regulating the expressions of P-gp and MRP1; therefore, it can be taken as a significant marker and target in tumor therapy.

Keywords

Caveolin-1 Multidrug resistance P-gp MRP1 Esophageal squamous cell carcinoma 

Notes

Acknowledgments

We thank Jinfeng Miao, Lei Cao, Qian Wu, Xiangyong Tian, Tao Wang, and Yalan Jiang for their suggestions and technical assistance. This work was supported by the National Natural Science Foundation of China (No. 81101686) and Foundation of He’nan Educational Committee (Nos. 12A310008 and 15A310029).

Compliance with ethical standards

Conflicts of interest

None

Supplementary material

13277_2015_4778_MOESM1_ESM.doc (687 kb)
ESM 1 (DOC 687 kb)
13277_2015_4778_MOESM2_ESM.doc (602 kb)
ESM 2 (DOC 602 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Song Zhang
    • 1
  • Wenbo Cao
    • 2
  • Mingjin Yue
    • 3
  • Naigang Zheng
    • 2
  • Tao Hu
    • 2
  • Shengli Yang
    • 2
    • 4
  • Ziming Dong
    • 2
    • 4
  • Shixin Lu
    • 1
  • Saijun Mo
    • 2
    • 4
  1. 1.The First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Department of Basic Science of Oncology, School of Basic Medical SciencesZhengzhou UniversityZhengzhouChina
  3. 3.Henan Tianxing Education and Media Company, LimitedZhengzhouChina
  4. 4.Collaborative Innovation Center of Henan Province for Cancer ChemopreventionZhengzhouChina

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