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Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest

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Tumor Biology

Abstract

Recently, a lectin was purified from the potato cultivated in Bangladesh locally known as Sheel. In the present study cytotoxicity of the lectin against Ehrlich ascites carcinoma (EAC) cells was studied by MTT assay in vitro in RPMI-1640 medium and 8.0–36.0 % cell growth inhibition was observed at the range of 2.5–160 μg/ml protein concentration when incubated for 24 h. The lectin-induced apoptosis in EAC cells was confirmed by fluorescence and optical microscope. The apoptotic cell death was also confirmed by using caspase inhibitors. Cells growth inhibition caused by the lectin (36 %) was remarkably decreased to 7.6 and 22.3 % respectively in the presence of caspase-3 and -8 inhibitors. RT-PCR was used to evaluate the expression of apoptosis-related genes Bcl-X, p53, and Bax. An intensive expression of Bcl-X gene was observed in untreated control EAC cells with the disappeared of the gene in Sheel-treated EAC cells. At the same time, Bax gene expression appeared only in Sheel-treated EAC cells and the expression level of the p53 gene was increased remarkable after the treatment of EAC cells with the lectin. The lectin showed strong agglutination activity against EAC cells. Flow cytometry was used to study the cell cycle phases of EAC cells and it was observed that the lectin arrested the G2/M phase. In conclusion, Sheel lectin inhibited EAC cells growth by inducing apoptosis.

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References

  1. Hasan I, Islam F, Ozeki Y, Kabir SR. Antiproliferative activity of cytotoxic tuber lectins from Solanum tuberosum against experimentally induced Ehrlich ascites carcinoma in mice. African J of Biotechnol. 2014;13:1679–85.

    Article  CAS  Google Scholar 

  2. Ferriz-Martinez RA, Torres-Arteaga IC, Blanco-Labra A, Garcia-Gasca T. New Approaches in the Treatment of Cancer. New York: Nova Science; 2010. p. 71–89.

    Google Scholar 

  3. Kabir SR, Hasan I, Zubair MA. Lectins from Medicinal Plants: Characterizations and Biological Properties. In: Recent Progress in Medicinal Plants-Nutraceuticals & Functional Food (Vol 42). USA: Studium Press LLC; 2015. p. 339–56.

    Google Scholar 

  4. Lin P, Ye X, Ng T. Purification of melibiose-binding lectins from two cultivars of Chinese black soybeans. Acta Biochim Biophys Sin (Shanghai). 2008;40:1029–38.

    Article  CAS  Google Scholar 

  5. Liu B, Bian HJ, Bao JK. Plant lectins: potential antineoplastic drugs from bench to clinic. Cancer Lett. 2010;287:1–12.

    Article  CAS  PubMed  Google Scholar 

  6. Kabir SR, Nabi MM, Haque A, Zaman RU, Mahmud ZH, Reza MA. Pea lectin inhibits growth of Ehrlich ascites carcinoma cells by inducing apoptosis and G2/M cell cycle arrest in vivo in mice. Phytomedicine. 2013;20:1288–96.

    Article  CAS  PubMed  Google Scholar 

  7. Liu B, Li CY, Bian HJ, Min MW, Chen LF, Bao JK. Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells. Arch Biochem Biophys. 2009;482:1–6.

    Article  CAS  PubMed  Google Scholar 

  8. Zhang CZ, Fang EF, Zhang HT, Liu LL, Yun JP. Momordica charantia lectin exhibits antitumor activity towards hepatocellular carcinoma. Invest New Drugs. 2015;33:1–11.

    Article  CAS  PubMed  Google Scholar 

  9. Panda PK, Mukhopadhyay S, Behera B, Bhol CS, Dey S, Das DN, et al. Antitumor effect of soybean lectin mediated through reactive oxygen species-dependent pathway. Life Sci. 2014;111:27–35.

    Article  CAS  PubMed  Google Scholar 

  10. Silva Fde O, Santos P, Figueirôa Ede O, de Melo CM, de Andrade Lemoine Neves JK, Arruda FV, et al. Antiproliferative effect of Canavalia brasiliensis lectin on B16F10 cells. Res Vet Sci. 2014;96:276–82.

    Article  CAS  PubMed  Google Scholar 

  11. Shi Z, Chen J, Li CY, An N, Wang ZJ, Yang SL, et al. Antitumor effects of concanavalin A and Sophora flavescens lectin in vitro and in vivo. Acta Pharmacol Sin. 2014;35:248–56.

    Article  CAS  PubMed  Google Scholar 

  12. Zhou W, Gao Y, Xu S, Yang Z, Xu T. Purification of a mannose-binding lectin Pinellia ternata agglutinin and its induction of apoptosis in Bel-7404 cells. Protein Expr Purif. 2014;93:11–7.

    Article  CAS  PubMed  Google Scholar 

  13. Hasan I, Ozeki Y, Kabir SR. Purification of a novel chitin-binding lectin with antimicrobial and antibiofilm activities from a Bangladeshi cultivar of potato (Solanum tuberosum). Indian J Biochem Biophys. 2014;51:142–8.

    CAS  PubMed  Google Scholar 

  14. Islam F, Khanam JA, Khatun M, Zuberi N, Khatun L, Kabir SR, et al. P-menth-1-ene-4, 7-diol from Eucalyptus camaldulensis Dhnh. inhibitEhrlich Ascites Carcinoma (EAC) cells growth by apoptosis and G2/M cell cycle arrest. Phytother Res. 2015;29:573–81.

    Article  CAS  PubMed  Google Scholar 

  15. Chen L, Watkins JF. Evidence against the presence of H2 histocompatibility antigens in Ehrlich ascites tumour cells. Nature. 1970;225(5234):734–5.

    Article  CAS  PubMed  Google Scholar 

  16. Ogawara M, Utsugi M, Yamazaki M, Sone S. Induction of human monocyte-mediated tumor cell killing by a plant lectin, wheat germ agglutinin. Jpn J Cancer Res (Gann). 1985;76:1107–14.

    CAS  Google Scholar 

  17. Ogawara M, Sone S, Ogura T. Human alveolar macrophages: wheat germ agglutinin-dependent tumor cell killing. Jpn J Cancer Res (Gann). 1987;78:288–95.

    CAS  Google Scholar 

  18. Suen YK, Fung KP, Choy YM, Lee CY, Chan CW, Kong SK. Concanavalin A induced apoptosis in murine macrophage PU5-1.8 cells through clustering of mitochondria and release of cytochrome c. Apoptosis. 2000;5:369–77.

    Article  CAS  PubMed  Google Scholar 

  19. Hostanska K, Vuong V, Rocha S, Soengas MS, Glanzmann C, Saller R, et al. Recombinant mistletoe lectin induces p53-independent apoptosis in tumour cells and cooperates with ionising radiation. Br J Cancer. 2003;88:1785–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Kim MS, Lee J, Lee KM, Yang SH, Choi S, Chung SY, et al. Involvement of hydrogen peroxide in mistletoe lectin-II-induced apoptosis of myeloleukemic U937 cells. Life Sci. 2003;73:1231–43.

    Article  CAS  PubMed  Google Scholar 

  21. Miyoshi N, Koyama Y, Katsuno Y, Hayakawa S, Mita T, Ohta T, et al. Apoptosis induction associated with cell cycle dysregulation by rice bran agglutinin. J Biochem. 2001;130:799–805.

    Article  CAS  PubMed  Google Scholar 

  22. Wang H, Ng TB, Ooi VE, Liu WK. Effects of lectins with different carbohydrate-binding specificities on hepatoma, choriocarcinoma, melanoma and osteosarcoma cell lines. Int J Biochem Cell Biol. 2000;32:365–72.

    Article  CAS  PubMed  Google Scholar 

  23. Zhang ZT, Peng H, Li CY, Liu JJ, Zhou TT, Yan YF, et al. Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis via a caspase-dependent pathway as compared to Ophiopogon japonicus lectin. Phytomedicine. 2010;18:25–31.

    Article  CAS  PubMed  Google Scholar 

  24. Kabir SR, Reza MA. Antibacterial activity of Kaempferia rotunda rhizome lectin and its induction of apoptosis in Ehrlich ascites carcinoma cells. App Biochem Biotech. 2014;172:2866–76.

    Article  CAS  Google Scholar 

  25. Gradilone A, Gazzaniga P, Ribuffo D, Scarpa S, Cigna E, Vasaturo F, et al. Survivin, bcl-2, bax, and bcl-X gene expression in sentinel lymph nodes from melanoma patients. J Clin Oncol. 2003;21:306–12.

    Article  CAS  PubMed  Google Scholar 

  26. Yan Q, Li Y, Jiang Z, Sun Y, Zhu L, Ding Z. Antiproliferation and apoptosis of human tumor cell lines by a lectin (AMML) of Astragalus mongholicus. Phytomedicine. 2009;16:586–93.

    Article  CAS  PubMed  Google Scholar 

  27. Bhattacharjee P, Giri B, Gomes A. Apoptogenic activity and toxicity studies of a cytotoxic protein (BMP1) from the aqueous extract of common Indian toad (Bufo melanostictus Schneider) skin. Toxicon. 2011;57:225–36.

    Article  CAS  PubMed  Google Scholar 

  28. Zhao Q, Cao X, Zeng B, Wang C, Yan L, Xu C. Musca domestica larva lectin induces apoptosis in BEL-7402 cells through a mitochondria-mediated reactive oxygen species way. Biol Pharm Bull. 2010;33:1274–8.

    Article  CAS  PubMed  Google Scholar 

  29. Kabir SR, Nabi MM, Nurujjaman M, Haque A, Mahmud ZH, Reza MA, et al. Momordica charantia seeds lectin: toxicity, bacterial agglutination and antitumor properties. App Biochem Biotech. 2015;175:2616–28.

    Article  CAS  Google Scholar 

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Acknowledgement

This research work was funded by the Faculty of Science, Rajshahi University and Ministry of Science and Technology (Grant No. 39.009.002.01.00.053.2014-2015/MEDI’S-165), Bangladesh.

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Correspondence to Syed Rashel Kabir.

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Kabir, S.R., Rahman, M.M., Amin, R. et al. Solanum tuberosum lectin inhibits Ehrlich ascites carcinoma cells growth by inducing apoptosis and G2/M cell cycle arrest. Tumor Biol. 37, 8437–8444 (2016). https://doi.org/10.1007/s13277-015-4735-x

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  • DOI: https://doi.org/10.1007/s13277-015-4735-x

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