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Tumor Biology

, Volume 37, Issue 6, pp 8239–8248 | Cite as

MiR-214 suppressed ovarian cancer and negatively regulated semaphorin 4D

  • Yang Liu
  • Honglin Zhou
  • Lan Ma
  • Youfang Hou
  • Jing Pan
  • Chunyi Sun
  • Yingying Yang
  • Jie Zhang
Original Article

Abstract

Ovarian cancer is one of the most common human malignancies in women. MiR-214 and semaphorin 4D (sema 4D) were found to be abhorrently expressed and involved in the progress of several kinds of malignant cancers. This study is aimed to investigate the cellular role of miR-214 and demonstrate that miR-214 negatively regulated sema 4D in ovarian cancer cells. The data showed that miR-214 expression was consistently lower in ovarian cancer tissues and cells than those in the normal controls. Over-expression of miR-214 in ovarian cancer SKOV-3 cells inhibited cell proliferation and induced apoptosis. It was suggested that miR-214 functioned as the tumor suppressor in ovarian cancer. Bioinformatic analysis indicated that miR-214 possibly regulated sema 4D by binding the sema 4D messenger RNA (mRNA) 3′-untranslated region (UTR). Sema 4D mRNA and protein levels were up-regulated in ovarian cancer tissues and SKOV-3 cells. Up-regulation of miR-214 in SKOV-3 cell line suppressed the sema 4D expression in both protein and nucleic acid levels. While, down-regulation of miR-214 in SKOV-3 cells would increase sema 4D protein and nucleic acid expression levels. The effects of miR-214 up- and down-regulation on luciferase activities of wild-type (WT) sema 4D 3′-UTR were completely removed upon introduction of mutation in 3′-UTR of WT sema 4D. Therefore, the data also demonstrated that sema 4D was the direct target of miR-214 and was negatively regulated by miR-214 in ovarian cancer cells.

Keywords

MiR-214 Semaphorin 4D Expression Negative regulation Ovarian cancer 

Abbreviations

Sema 4D

Semaphorin 4D

FBS

Fetal bovine serum

3′-UTR

3′-Untranslated region

LZTS1

Leucine zipper putative tumor suppressor 1

DMEM

Dulbecco’s modified Eagle’s medium

RPMI

Roswell Park Memorial Institute

PVDF

Polyvinylidene fluoride

Notes

Acknowledgments

This work was financially sponsored by Key Project of Educational Department in Yunnan Province (Nos. 2014Z075 and 2015Z081).

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yang Liu
    • 2
  • Honglin Zhou
    • 2
  • Lan Ma
    • 3
  • Youfang Hou
    • 2
  • Jing Pan
    • 2
  • Chunyi Sun
    • 2
  • Yingying Yang
    • 2
  • Jie Zhang
    • 1
  1. 1.Department of General SurgeryThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina
  2. 2.Department of GynecologyThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina
  3. 3.Department of Reproduction and GeneticThe Second Affiliated Hospital of Kunming Medical UniversityKunmingChina

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