Tumor Biology

, Volume 37, Issue 7, pp 8799–8809 | Cite as

PIK3CA and PIK3CB silencing by RNAi reverse MDR and inhibit tumorigenic properties in human colorectal carcinoma

  • Shuhua Wu
  • Feifei Wen
  • Yangyang Li
  • Xiangqian Gao
  • Shuang He
  • Mengyao Liu
  • Xiangzhi Zhang
  • Dong Tian
Original Article


Colorectal carcinoma (CRC) is the second most common and frequent cause of cancer-related deaths for men and women in the world. PIK3CA and PIK3CB that reverse multidrug resistance (MDR) can serve as predictive and prognostic markers as well as therapeutic targets for CRC treatment. In the present study, we showed that PIK3CA and PIK3CB are upregulated in CRCs and positively correlated with MDR-1, LRP, and GST-π. Long-term monitoring of 316 CRC patients showed that PIK3CA and PIK3CB were associated with poor survival time as shown by Kaplan-Meier analysis. Furthermore, we found that the downregulation of PIK3CA and PIK3CB reversed MDR; inhibited the capability of proliferation, migration, and invasion of CRC cells; and slowed down the CRC tumor growth in nude mice. Consistent with clinical observations, PIK3CA and PIK3CB significantly increase multidrug resistance of CRC cells in vivo. Together, these results suggest that PIK3CA and PIK3CB may be used as potential therapeutic drug targets for colorectal cancer.


Colorectal cancer PIK3CA PIK3CB Stably transfected cell lines MDR Gene therapy 



This work was supported by the Scientific and Technological Project of Shandong Province.

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Shuhua Wu
    • 1
  • Feifei Wen
    • 1
  • Yangyang Li
    • 1
  • Xiangqian Gao
    • 1
  • Shuang He
    • 1
  • Mengyao Liu
    • 1
  • Xiangzhi Zhang
    • 1
  • Dong Tian
    • 1
  1. 1.The Department of PathologyBinzhou Medical University HospitalBinzhouChina

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