Tumor Biology

, Volume 37, Issue 6, pp 7929–7937 | Cite as

Active cigarette smoking and the risk of breast cancer at the level of N-acetyltransferase 2 (NAT2) gene polymorphisms

  • Petra KasajovaEmail author
  • Veronika Holubekova
  • Andrea Mendelova
  • Zora Lasabova
  • Pavol Zubor
  • Erik Kudela
  • Kristina Biskupska-Bodova
  • Jan Danko
Original Article


The aim of our study was to assess the correlation between the tobacco exposure and NAT2 gene (rs1041983 C/T, rs1801280 T/C, rs1799930 G/A) polymorphisms in association with breast cancer development. We wanted to determine the prognostic clinical importance of these polymorphisms in association with smoking and breast cancer. For the detection of possible association between smoking, NAT2 gene polymorphisms, and the risk of breast cancer, we designed a case-controlled study with 198 patients enrolled, 98 breast cancer patients and 100 healthy controls. Ten milliliters of peripheral blood from the cubital vein was withdrawn from every patient. The HRM (high resolution melting) analysis was used for the detection of three abovementioned NAT2 gene polymorphisms. When comparing a group of women smoking more than 5 cigarettes a day with the patients smoking fewer than 5 cigarettes a day, we found out that if women were the carriers of aberrant AA genotype for rs1799930, the first group of women had higher risk of breast carcinoma than the second group. If patients were the carriers of aberrant TT genotype for rs1041983, for rs1801280CC genotype, and rs1799930AA genotype and they smoked more than 5 cigarettes a day, they had higher risk of malignant breast disease than never-smoking women. Our results confirm the hypothesis that NAT2 gene polymorphisms (rs1041983 C/T, rs1801280 T/C, and rs1799930 G/A) in association with long-period active smoking could be the possible individual risk-predicting factors for breast cancer development in the population of Slovak women.


Smoking Breast cancer NAT2 gene Polymorphisms 



This work was supported by the project “Centre of excellence for perinatology research (CEPV I),” ITMS:26220120016, co-financed by EU grants, by the project “Centre of excellence for perinatology research establishment (CEPV II)”, ITMS: 26220120036, co-financed by EU grants, University grant UK/468/2011, UK/372/2012, UK/116/2013, by project VEGA MŠ1/0243/12 and APVV-14-0815.

Compliance with ethical standards


This study was funded by the project “Centre of excellence for perinatology research (CEPV I)”, ITMS:26220120016, co-financed by EU grants, by the project “Centre of excellence for perinatology research establishment (CEPV II)”, ITMS: 26220120036, co-financed by EU grants, University grant UK/468/2011, UK/372/2012, UK/116/2013, by project VEGA MŠ1/0243/12 and APVV-14-0815.

Conflict of interest


Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Petra Kasajova
    • 1
    Email author
  • Veronika Holubekova
    • 2
  • Andrea Mendelova
    • 2
  • Zora Lasabova
    • 2
  • Pavol Zubor
    • 1
  • Erik Kudela
    • 1
  • Kristina Biskupska-Bodova
    • 1
  • Jan Danko
    • 1
  1. 1.Department of Obstetrics and Gynecology, Jessenius Faculty of MedicineComenius University in BratislavaMartinSlovakia
  2. 2.Biomedical Center MartinComenius University in BratislavaMartinSlovakia

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