Functional characterization of TRPM7 in nasopharyngeal carcinoma and its knockdown effects on tumorigenesis
The aim of this study was to evaluate the association of functional expression of TRPM7 with nasopharyngeal carcinoma (NPC) growth. We examined the correlation of TRPM7 expression with cell growth and proliferation, cell cycle, and apoptosis in vitro in NPC cell lines and NPC tumorigenesis in mice by conducting experiments in mice and by further analyzing the tumor volume and growth. We further explored to see whether there is any positive correlation with the TRPM7 knockdown in NPC cells with their sensitivity to radiation. We found that the functional expression of TRPM7 in nasopharyngeal carcinoma is a critical requirement for physiological processes such as cell cycle, resistance to apoptosis, and cell proliferation. TRPM7 knockdown also enhanced sensitivity to radiotherapy of nasopharyngeal carcinoma. Moreover, we identified TRPM7 as a novel potential regulator of cell proliferation in NPC, through signal transducer and activator of transcription 3 (STAT3)-mediated signaling pathway and other anti-apoptotic factors. TRPM7 and STAT3 activation might be critical for the growth of NPC cells and could be an effective target for treatment of nasopharyngeal carcinoma.
KeywordsNasopharyngeal carcinoma TRPM7 Prognosis Apoptosis CNE-1 cells STAT3 Radiotherapy
This study was supported by the Natural Science Foundation of Guangdong Province, China (S2013040014989) and China Postdoctoral Science Foundation (2015M570742).
XX. W and BQ. L conceived and designed the study and critically revised the manuscript.
Y. Q, ZW. L, and JY. L performed the experiments, analyzed the data, and drafted the manuscript.
WZ. W, AS. L, and PX. S helped in study design, study implementation, and manuscript revision.
All authors read and approved the final manuscript.
Compliance with ethical standards
Conflicts of interest
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