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Tumor Biology

, Volume 37, Issue 6, pp 7981–7987 | Cite as

MiR-130b inhibits proliferation and induces apoptosis of gastric cancer cells via CYLD

  • Baoyou Sun
  • Lei Li
  • Wendong Ma
  • Shikang Wang
  • Chunjin Huang
Original Article

Abstract

A role of microRNA-130b (miR-130b) in the carcinogenesis of gastric cancer remains undetermined. In this study, we studied the effects and mechanism of miR-130b to the gastric cell proliferation and apoptosis. We found that the levels of miR-130b significantly up-regulated in gastric cancer tissue, compared to the paired adjacent non-tumor gastric tissue. The miR-130b levels in gastric cancer cell lines were significantly higher than those in control normal gastric tissues. Transfection with the miR-130b mimic enhanced the cell proliferation and suppressed cell apoptosis in gastric cancer cells, while transfection with the anti-sense of miR-130b (anti-miR-130b) suppressed cell proliferation and induced cell apoptosis in gastric cancer cells. Bioinformatics analyses showed that cylindromatosis gene (CYLD) was a potential target gene of miR-130b. The luciferase activity assay and western blot verified that miR-130b targeted CYLD messenger RNA (mRNA) to modulate its protein levels. Together, our study suggests that aberrantly expressed miR-130b may regulate cell apoptosis and proliferation of human gastric cancer cells via CYLD, which appears to be a promising therapeutic target for gastric cancer.

Keywords

Gastric cancer miR-130b CYLD Proliferation Apoptosis 

Notes

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Baoyou Sun
    • 1
  • Lei Li
    • 1
  • Wendong Ma
    • 1
  • Shikang Wang
    • 1
  • Chunjin Huang
    • 2
  1. 1.Shandong Provincial Hospital Affiliated to Shandong UniversityJinanChina
  2. 2.Department of General SurgeryHuadong Hospital of Fudan UniversityShanghaiChina

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