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Tumor Biology

, Volume 37, Issue 5, pp 6923–6933 | Cite as

Synthesis and antitumor activity evaluation of a novel porphyrin derivative for photodynamic therapy in vitro and in vivo

  • Li-Jun Zhang
  • Yi-Jia Yan
  • Ping-Yong Liao
  • Davor Margetic
  • Li Wang
  • Zhi-Long Chen
Original Article

Abstract

A novel porphyrin derivative, 5, 10, 15, 20-tetrakis (5-morpholinopentyl)-21H, 23H-Porphin (MPP, 4) and its photophysical characteristics, therapeutic efficacy of photodynamic therapy (PDT) in vitro and in vivo, tumor selectivity, and clearance from normal tissues were investigated here. MPP has strong absorption at relatively long wavelength (λmax = 648 nm, molar absorption coefficient ε ∼ 17,200 M−1cm−1) and can emit strong fluorescence at 653 and 718 nm. When administered to the animal tumor models by tail vein injection, MPP was capable of accumulating in the tumor site, as examined in vivo with the fluorescence signal of MPP. By the combination of MPP and a 650-nm laser irradiation, the viability of T24 cells could decrease by 4.37 %, and inhibition rate of T24 tumor could increase up to 91.21 % compared with control group, demonstrating the potential of MPP as an effective photosensitizer in PDT for tumor treatment.

Keywords

MPP Porphyrin Antitumor Photodynamic therapy Photosensitizer 

Abbreviations

MPP 5

10, 15, 20-tetrakis (5-morpholinopentyl)-21H, 23H-Porphin

PDT

Photodynamic therapy

ros

Reactive oxygen species

O2

Singlet oxygen

DPBF

1, 3-diphenylisobenzofuran

DMSO

Dimethyl sulfoxide

DMF

N, N-dimethylformamide

PBS

Phosphate buffered saline

T24

Cell human bladder cancer cell line

FBS

Fetal bovine serum

H&E

Hematoxylin-eosin

MTT

3-(4, 5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium bromide

Notes

Acknowledgments

This work was supported by Chinese National Natural Science Foundation (No. 21372042, 21402236, 81301878), Foundation of Shanghai government (No.14431906200, 14140903500, 13431900700, 13430722300, 13ZR1441000, 13ZR1440900, 14ZR1439800, 14ZR1439900, 15ZR1439900, 15XD1523400, 14SJGGYY08, 201370), International Cooperation Foundation of China and Croatia (6–11) and Foundation of Yiwu Science and Technology Bureau (No. 2012-G3-02, 2013-G3-03).

Compliance with ethical standards

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Li-Jun Zhang
    • 1
  • Yi-Jia Yan
    • 2
  • Ping-Yong Liao
    • 1
  • Davor Margetic
    • 3
  • Li Wang
    • 1
  • Zhi-Long Chen
    • 1
  1. 1.Department of Pharmaceutical Science & Technology, College of Chemistry and BiologyDonghua UniversityShanghaiChina
  2. 2.Ningbo Dongmi Pharmaceutical Co. LtdNingboChina
  3. 3.Division of organic chemistry and biochemistryRudjer Boskovic InstituteZagrebCroatia

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