Tumor Biology

, Volume 37, Issue 5, pp 6831–6836 | Cite as

SphK1 promotes tumor cell migration and invasion in colorectal cancer

  • Jianting Long
  • Ying Xie
  • Junmei Yin
  • Wei Lu
  • Shi Fang
Original Article


Colorectal cancer (CRC) is one of the most common cancers worldwide. Sphingosine kinase 1 (SphK1), which phosphorylates sphingosine to sphingosine-1-phosphate (S1P), is overexpressed in various types of cancers and may act as an oncogene in tumorigenesis. However, little is known about the role of SphK1 in CRC patients. We studied the expression of SphK1 in 85 cases of CRC tissues by immunohistochemistry, qRT-PCR, and western blot. We also evaluated the effect of SphK1 on cell proliferation and invasion by MTT and transwell invasion assay. SphK1 is overexpressed in CRC tissues and cell lines, and upregulation of SphK1 correlated significantly with the following parameters: lymph node metastasis, liver metastasis, and advanced TNM stage. SphK1 knockdown results in inhibition of cancer cell proliferation. Inhibition of CRC cell migration and invasion is also evident through reversal of EMT by increases in E-cadherin expression and decreases in vimentin expression. In conclusion, SphK1 is associated with the proliferation and invasiveness of CRC cells and the SphK1 gene may contribute to a novel therapeutic approach against CRC.


Colorectal cancer SphK1 Proliferation Invasion EMT Targeted therapy 



This work is supported by the Science and Technology Planning Project of Guangdong Province (2013B021800284)

Compliance with ethical standards

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Jianting Long
    • 1
  • Ying Xie
    • 2
  • Junmei Yin
    • 2
  • Wei Lu
    • 2
  • Shi Fang
    • 2
  1. 1.Department of Medicinal OncologyThe First Affiliated Hospital, Sun Yat-Sen UniversityGuangzhouChina
  2. 2.Department of Clinic Nutritionthe First Affiliated Hospital of Sun Yat-Sen UniversityGuangzhouChina

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