Tumor Biology

, Volume 37, Issue 5, pp 6511–6517 | Cite as

In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system

  • Fadwa Chaabane
  • Nadia Mustapha
  • Imen Mokdad-Bzeouich
  • Aicha Sassi
  • Soumaya Kilani-Jaziri
  • Marie-Geneviève Dijoux Franca
  • Serge Michalet
  • Mayssa Fathallah
  • Mounira Krifa
  • Kamel Ghedira
  • Leila Chekir-Ghedira
Original Article


The purpose of this study was to assess the antitumor and immunomodulatory effects of the aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells and treated intraperitoneally with the aqueous extract at 200 mg/Kg b.w for 21 days. After euthanization on day 22, the tumors were weighed; lymphocyte proliferation, cytotoxic T lymphocyte (CTL), and natural killer (NK) cell activities were evaluated using the MTT assay. Macrophage phagocytosis was studied by measuring the lysosomal activity. In addition to its potential to inhibit the growth of the transplantable tumor, the aqueous extract remarkably induced splenocyte proliferation and both NK and CTL activities in tumor-bearing mice. The aqueous extract was also seen to have promoted lysosomal activity of host macrophages.


Daphne gnidium Anti-melanoma Immunomodulation B16-F10 cells Keratinocytes 



We acknowledge the financial support from the Ministry of Higher Education and Scientific Research, Tunisia.

Compliance with ethical standards

All experiments were performed in accordance with guidelines for the care and use of laboratory animals as published by the US National Institute of Health. All experiments received the explicit approval of the Ethics Animal Committee in Tunisia.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Fadwa Chaabane
    • 1
  • Nadia Mustapha
    • 1
  • Imen Mokdad-Bzeouich
    • 1
  • Aicha Sassi
    • 1
  • Soumaya Kilani-Jaziri
    • 1
  • Marie-Geneviève Dijoux Franca
    • 3
  • Serge Michalet
    • 3
  • Mayssa Fathallah
    • 1
  • Mounira Krifa
    • 1
  • Kamel Ghedira
    • 1
  • Leila Chekir-Ghedira
    • 1
    • 2
  1. 1.Unité de substances naturelles bioactives et biotechnologyFaculté de pharmacie de MonastirMonastirTunisia
  2. 2.Laboratoire de biologie cellulaire et moléculaireFaculté de médecine dentaire de MonastirMonastirTunisia
  3. 3.UMR 5557 CNRS/Université Lyon1, Equipe Multirésistance environnementale et efflux bactérienCentre d’étude des substances naturellesLyonFrance

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