Advertisement

Tumor Biology

, Volume 37, Issue 5, pp 5897–5903 | Cite as

The involvement of hematopoietic pre-B cell leukemia transcription factor-interacting protein in regulating epithelial-mesenchymal transition of human spinal glioblastoma

  • Deliang Wang
  • Li Wang
  • Yi Zhou
  • Xinjun Zhao
  • Hui Xiong
Original Article

Abstract

To date, hematopoietic pre-B cell leukemia transcription factor-interacting protein (HPIP), a co-repressor for the transcription factor PBX, has been involved into the initiation and onset in a wide variety of cancers. However, the molecular mechanisms underlying HPIP-induced epithelial-mesenchymal transition (EMT) in the spinal glioblastoma have been under investigation. In the present study, spinal glioblastoma tissues, U87, and U251 cell lines were used and subjected to in vitro assays, such as RT-PCR, and Western blot. Here, in vitro assays revealed that HPIP mRNA and protein were highly expressed in five cases of spinal glioblastoma tissues, compared with non-tumor tissues. Subsequently, in vitro experiments demonstrated HPIP promoted the U87 and U251 cell growth and regulated the G1/S phase transitions in U87 and U251 cell cycle, respectively, accompanied by the increased expression of cyclin A2, cyclin B1, and cyclin D1. Furthermore, HPIP increased the expression of N-cadherin, Slug, and MMP2, and decreased the expression of E-cadherin. By contrast, knockdown of HPIP reversed HPIP-induced EMT biomarkers, migration, and invasion in U87 and U251 cells. In conclusion, our findings identified HPIP plays an important role in the progression and EMT of spinal glioblastoma, by which cell growth is improved. Thus, HPIP gene or protein could act as a useful target in the clinical practice.

Keywords

EMT Glioblastoma HPIP Regulation 

Notes

Acknowledgments

We greatly thank other members of our lab for the valuable suggestions and writing.

Compliance with ethical standards

Conflicts of interest

None

References

  1. 1.
    Bouffet E, Pierre-Kahn A, Marchal JC, et al. Prognostic factors in spinal cord astrocytoma. Cancer. 2000;83:2391–9.CrossRefGoogle Scholar
  2. 2.
    Govindan A, Chakraborti S, Mahadevan A, et al. Histopathologic and immunohistochemical profile of spinal glioblastoma: a study of six cases. Brain Tumor Pathol. 2011;28(4):297–303.CrossRefPubMedGoogle Scholar
  3. 3.
    Henson JW. Spinal cord gliomas. Curr Opin Neurol. 2001;14:679–82.CrossRefPubMedGoogle Scholar
  4. 4.
    Liu X, Germin BI, Ekholm S. A case of cervical spinal cord glioblastoma diagnosed with MR diffusion tensor and perfusion imaging. J Neuroimaging. 2011;21(3):292–6.CrossRefPubMedGoogle Scholar
  5. 5.
    Jallo GI, Freed D, Epstein F. Intramedullary spinal cord tumors in children. Childs Nerv Syst. 2003;19:641–9.CrossRefPubMedGoogle Scholar
  6. 6.
    Houten JK, Cooper PR. Spinal cord astrocytomas: presentation, management and outcome. J Neurooncol. 2000;47:219–24.CrossRefPubMedGoogle Scholar
  7. 7.
    Abramovich C, Chavez EA, Lansdorp PM, et al. Functional characterization of multiple domains involved in the subcellular localization of the hematopoietic Pbx interacting protein (HPIP). Oncogene. 2002;21:6766–71.CrossRefPubMedGoogle Scholar
  8. 8.
    Abramovich C, Shen WF, Pineault N, et al. Functional cloning and characterization of a novel nonhomeodomain protein that inhibits the binding of PBX1-HOX complexes to DNA. J Biol Chem. 2000;275:26172–7.CrossRefPubMedGoogle Scholar
  9. 9.
    Manavathi B, Acconcia F, Rayala SK, et al. An inherent role of microtubule network in the action of nuclear receptor. Proc Natl Acad Sci U S A. 2006;103:15981–6.CrossRefPubMedPubMedCentralGoogle Scholar
  10. 10.
    Wang X, Yang Z, Zhang H, et al. The estrogen receptor-interacting protein HPIP increases estrogen-responsive gene expression through activation of MAPK and AKT. Biochim Biophys Acta. 2008;2008:1220–8.CrossRefGoogle Scholar
  11. 11.
    Santi M, Mena H, Wong K, et al. Spinal cord malignant astrocytomas. Clinicopathologic features in 36 cases. Cancer. 2003;98:554–61.CrossRefPubMedGoogle Scholar
  12. 12.
    Kim MS, Chung CK, Choe G, et al. Intramedullary spinal cord astrocytoma in adults: postoperative outcome. J Neurooncol. 2001;52(1):85–94.CrossRefPubMedGoogle Scholar
  13. 13.
    Fang C, Shen Y, Qi P, et al. Astrocyte-elevated gene-1 mediates insulin-like growth factor 1-induced the progression of cardiac myxoma. Tumour Biol. 2015 Jul 10. [Epub ahead of print]Google Scholar
  14. 14.
    Lv B, Yang X, Lv S, et al. CXCR4 signaling induced epithelial-mesenchymal transition by PI3K/AKT and ERK pathways in glioblastoma. Mol Neurobiol. 2015;52(3):1263–8.CrossRefPubMedGoogle Scholar
  15. 15.
    Li J, Li H, Liu J, et al. The Clinical Implications of Human Telomerase Reverse Transcriptase Expression in Grade and Prognosis of Gliomas: a Systematic Review and Meta-analysis. Mol Neurobiol. 2015 Apr 21. [Epub ahead of print]Google Scholar
  16. 16.
    Yao C, Li P, Song H, et al. CXCL12/CXCR4 Axis Upregulates Twist to Induce EMT in Human Glioblastoma. Mol Neurobiol. 2015 Jul 16. [Epub ahead of print]Google Scholar
  17. 17.
    Han G, Wu D, Yang Y, et al. CrkL meditates CCL20/CCR6-induced EMT in gastric cancer. Cytokine. 2015;76(2):163–9.CrossRefPubMedGoogle Scholar
  18. 18.
    Yang X, Lv S, Zhou X, et al. The clinical implications of transforming growth factor beta in pathological grade and prognosis of glioma patients: a meta-analysis. Mol Neurobiol. 2015;52(1):270–6.CrossRefPubMedGoogle Scholar
  19. 19.
    Yang X, Lv S, Liu Y, et al. The clinical utility of matrix metalloproteinase 9 in evaluating pathological grade and prognosis of glioma patients: a meta-analysis. Mol Neurobiol. 2015;52(1):38–44.CrossRefPubMedGoogle Scholar

Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Deliang Wang
    • 1
  • Li Wang
    • 2
  • Yi Zhou
    • 1
  • Xinjun Zhao
    • 1
  • Hui Xiong
    • 1
  1. 1.Department of NeurosurgeryShandong University Hospital of Traditional Chinese MedicineJinanChina
  2. 2.Department of GynaecologyShandong University Hospital of Traditional Chinese MedicineJinanChina

Personalised recommendations