Aspirin acetylates wild type and mutant p53 in colon cancer cells: identification of aspirin acetylated sites on recombinant p53
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Aspirin’s ability to inhibit cell proliferation and induce apoptosis in cancer cell lines is considered to be an important mechanism for its anti-cancer effects. We previously demonstrated that aspirin acetylated the tumor suppressor protein p53 at lysine 382 in MDA-MB-231 human breast cancer cells. Here, we extended these observations to human colon cancer cells, HCT 116 harboring wild type p53, and HT-29 containing mutant p53. We demonstrate that aspirin induced acetylation of p53 in both cell lines in a concentration-dependent manner. Aspirin-acetylated p53 was localized to the nucleus. In both cell lines, aspirin induced p21CIP1. Aspirin also acetylated recombinant p53 (rp53) in vitro suggesting that it occurs through a non-enzymatic chemical reaction. Mass spectrometry analysis and immunoblotting identified 10 acetylated lysines on rp53, and molecular modeling showed that all lysines targeted by aspirin are surface exposed. Five of these lysines are localized to the DNA-binding domain, four to the nuclear localization signal domain, and one to the C-terminal regulatory domain. Our results suggest that aspirin’s anti-cancer effect may involve acetylation and activation of wild type and mutant p53 and induction of target gene expression. This is the first report attempting to characterize p53 acetylation sites targeted by aspirin.
KeywordsAspirin Acetylation p53 Apoptosis Anti-cancer effects
Supports from the Translational Cancer Research Seed Grant, funded as 2010 Research Initiative Center by the State of South Dakota, Faculty Excellence Fund from South Dakota State University, and from NIH (5RO3CA133061-02) to GJB are gratefully acknowledged. We also thank Raghavender Chivukula, Texas Tech University Health Science Center for helpful discussions and Dr. Fred Hagen, University of Rochester Medical Center, Rochester, NY, for carrying out MS analysis. Support to D. Ramesh Kumar to conduct molecular docking studies from the Department of Aquatic animal health division, CIBA, Chennai, India, is also gratefully acknowledged.
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- 19.Ai G, Dachineni R, Muley P, Tummala H, Bhat GJ. Aspirin and salicylic acid decrease c-myc expression in cancer cells: a potential role in chemoprevention. Tumour biology: the journal of the International Society for Oncodevelopmental Biology and Medicine 2015;1–12.Google Scholar
- 23.Alfonso L, Ai G, Spitale RC, Bhat GJ. Molecular targets of aspirin and cancer prevention. British journal of cancer 2014Google Scholar
- 24.Dovizio M, Bruno A, Tacconelli S, Patrignani P. Mode of action of aspirin as a chemopreventive agent. Recent results in cancer research Fortschritte der Krebsforschung Progres dans les recherches sur le cancer 2013;191:39–65.Google Scholar
- 31.Huang L, Wong CC, Cheng KW, Rigas B. Phospho-aspirin-2 (mdc-22) inhibits estrogen receptor positive breast cancer growth both in vitro and in vivo by a redox-dependent effect. 2014.Google Scholar
- 33.O’Connor PM, Jackman J, Bae I, Myers TG, Fan S, Mutoh M, et al. Characterization of the p53 tumor suppressor pathway in cell lines of the national cancer institute anticancer drug screen and correlations with the growth-inhibitory potency of 123 anticancer agents. Cancer research. 1997;57:4285–300.PubMedGoogle Scholar
- 36.Alzate O, Parker CE, Mocanu V, Mocanu M, Dicheva N, Warren MR. Mass spectrometry for post-translational modifications. 2010.Google Scholar
- 38.Borthwick GM, Johnson AS, Partington M, Burn J, Wilson R, Arthur HM. Therapeutic levels of aspirin and salicylate directly inhibit a model of angiogenesis through a cox-independent mechanism. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2006;20:2009–16.CrossRefGoogle Scholar
- 54.Wang J, Zhang C-J, Zhang J, He Y, Lee YM, Chen S, Lim TK, Ng S, Shen H-M, Lin Q. Mapping sites of aspirin-induced acetylations in live cells by quantitative acid-cleavable activity-based protein profiling (qa-abpp). Scientific reports 2015;5.Google Scholar