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Tumor Biology

, Volume 37, Issue 4, pp 5633–5644 | Cite as

The oncogenetic role of stanniocalcin 1 in lung adenocarcinoma: a promising serum candidate biomarker for tracking lung adenocarcinoma progression

  • Yu-Zhen Du
  • Xiao-Hua Gu
  • Shao-Fei Cheng
  • Li Li
  • Hua Liu
  • Liu-Ping Hu
  • Feng Gao
Original Article

Abstract

Stanniocalcin 1 (STC1) is reported to functionally participate in the development of several cancers. However, the role of STC1 in the tumorigenesis and progression of lung adenocarcinoma remains to be fully elucidated. Here, we found that the average levels of serum STC1 were 5.47, 5.53, and 6.94 ng/mL (P = 0.0045) in the healthy subjects and patients with lung adenocarcinoma at tumor stages I–II and III–IV according to Union for International Cancer Control (UICC), respectively. Subsequently, the positive correlation between the STC1 expression level in lung adenocarcinoma tissues and tumor stages was confirmed by immunohistochemical staining assay. Additionally, studies in the STC1-overexpressing or STC1-silenced stable cell lines showed that STC1 increased cell proliferation by promoting G1/S transition in cell cycle progression via up-regulating cyclin B1 and cyclin E. Moreover, studies in the STC1-overexpressing or STC1-silenced stable cell lines also showed that STC1 inhibited cell apoptosis by up-regulating the expression of anti-apoptosis proteins Bcl-2 and Bcl-xl and down-regulating the expression of pro-apoptosis proteins Bax, Bak, and Bid via the activation of the ERK and JNK signaling pathway. In addition, neutralization of STC1 with monoclonal antibody significantly increased the apoptosis of A549 cells. Taken together, our findings strongly suggest that elevated expression of STC1 protein at the III–IV stage of lung adenocarcinoma promotes tumorigenesis of lung adenocarcinoma and positively associates with the cancer progression, which may be of potential value as tumor marker in clinical tracking lung adenocarcinoma progression.

Keywords

Stanniocalcin 1 Lung adenocarcinoma Tumorigenesis Cell proliferation Cell apoptosis 

Notes

Compliance with ethical standards

Funding

This study was funded by research grants from the Shanghai Hospital Development Center (SHDC12013903).

Conflicts of interest

None

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of Research Ethics Committee of Shanghai Sixth People’s Hospital and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yu-Zhen Du
    • 1
  • Xiao-Hua Gu
    • 2
  • Shao-Fei Cheng
    • 3
  • Li Li
    • 1
  • Hua Liu
    • 1
  • Liu-Ping Hu
    • 1
  • Feng Gao
    • 1
  1. 1.Department of Laboratory MedicineShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  2. 2.Department of RespirationShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina
  3. 3.Department of Cardiothoracic SurgeryShanghai Jiao Tong University Affiliated Sixth People’s HospitalShanghaiChina

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