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Tumor Biology

, Volume 37, Issue 7, pp 9441–9450 | Cite as

Long non-coding RNA tumor suppressor candidate 7 functions as a tumor suppressor and inhibits proliferation in osteosarcoma

Original Article

Abstract

Osteosarcoma is the most common malignant tumor of bone. Recent studies have proven long non-coding RNAs (lncRNAs) play important roles in the tumorigenesis and progression of cancer. However, few lncRNAs have been investigated in osteosarcoma. Here, we reported a novel lncRNA, tumor suppressor candidate 7 (TUSC7), was significantly downregulated in osteosarcoma tissues compared with paired non-tumor tissues and low expression of TUSC7 indicated poor survival (HR = 0.313, 95 % confidence interval (CI) 0.092–0.867) of osteosarcoma patients. Further analysis revealed that loss copy number of TUSC7 was correlated with low expression of TUSC7, and additionally, loss of TUSC7 copy number also indicated poor prognosis (HR = 3.994, 95 % CI 1.147–13.91) of osteosarcoma patients. Two osteosarcoma cell lines, HOS and MG63, were utilized to investigate biological function of TUSC7. Cell counting kit 8 (CCK-8) assay revealed that after silence of TUSC7, cell proliferation ability increased and the colony formation ability also increased. Further results showed that cell cycle was not affected by treatment of si-TUSC7, while the percentage of apoptotic cells decreased. Western blot showed that after silence of TUSC7, the proapoptotic Bcl2 expression was downregulated. Finally, we established xenograft tumor models in nude mice with MG63 cells. Compared with negative control group, silence of TUSC7 significantly promoted tumor growth in vivo. Thus, we demonstrated that TUSC7 could be a potential tumor suppressor in osteosarcoma.

Keywords

Osteosarcoma lncRNA TUSC7 Tumor suppressor Apoptosis 

Notes

Acknowledgments

This work has no funding.

Compliance with ethical standards

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2016

Authors and Affiliations

  • Menglin Cong
    • 1
  • Jianmin Li
    • 2
  • Rui Jing
    • 3
  • Zhenzhong Li
    • 4
  1. 1.Department of Orthopedic SurgeryShandong University School of MedicineJinanChina
  2. 2.Department of Orthopedic SurgeryQilu Hospital of Shandong UniversityJinanChina
  3. 3.Department of Radiologythe General Hospital of Chinese People’s Liberation ArmyBeijingChina
  4. 4.Department of AnatomyShandong University School of MedicineJinanChina

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