miR-122 inhibits cancer cell malignancy by targeting PKM2 in gallbladder carcinoma
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Gallbladder cancer (GBC) is one of the lethal diseases of digestive system. Increasing evidence prompt that microRNAs (miRs) might provide a novel therapeutical target for malignant disease. The antitumor effect of miR-122 to GBC is worth to be investigated. miR-122 expression level in GBC tissue sample and cell lines were assayed by qRT-PCR. miR-122 mimics were transfected for upregulation of miR-122 expression. Cell function was assayed by CCK8, flow cytometry, wound healing assay, migration assay, and invasion assay. The target genes of miR-122 were predicated by TargetScan online program and verified by western blot and luciferase report gene assay. miR-122 was decreased in GBC tissue and cell lines. The exogenous introduction of miR-122 exhibits multiple antitumor effect in GBC cell proliferation, invasion, and metastasis. Further studies revealed that the PKM2 was a regulative target of miR-122 in GBC cell. miR-122 also inhibits TGF-β-induced epithelium mesenchymal transformation of GBC cell by downregulating PKM2 expression. These findings suggest that miR-122 plays an important role in tumorigenesis of GBC through interfering PKM2, highlighting its usefulness as a potential therapeutic agent in GBC.
KeywordsmiR-122 Gallbladder carcinoma Malignant PKM2
Wei Lu and Yingbin Liu contributed to the funding/financial support and the conception and design of the study. Wei Lu, Yijian Zhang, and Linzhu Zhou completed the data analysis and interpretation. Wei Lu and Yijian Zhang did the major manuscript writing. All authors are responsible for the provision of study materials or patients, collection and assembly of data, and final approval of the manuscript.
Compliance with ethical standards
This study was supported by the China National High Technology Research and Development Program (863 Program) (No. 2012AA022606), National Natural Science Foundation of China (No. 91440203, 81172026, 81272402, 81301816, and 81172029), China Postdoctoral Science Foundation (No. 2014M561487), and Interdisciplinary Program of Shanghai JiaoTong University (No. 14JCRY05).
Conflicts of interest
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