Tumor Biology

, Volume 37, Issue 5, pp 5869–5878 | Cite as

Sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway

  • Yue-Can Zeng
  • Rui Xing
  • Jing Zeng
  • Ming Xue
  • Feng Chi
  • Yan Xin
  • Guo-Liang Fan
  • Hong-Mei Wang
  • Qiong-Yu Duan
  • Yu-Nan Sun
  • Nan Niu
  • Rong Wu
Original Article


The purpose of this study was to evaluate the radiation-enhancing effect of sodium glycididazole, and the corresponding mechanisms of action on laryngeal cancer cells. Two laryngeal cancer cell lines (Hep-2 and UT-SCC-19A) were irradiated with X-rays in the presence or absence of sodium glycididazole. Cell survival, DNA damage and repair, cell apoptosis, cell cycle distribution, expression of proteins related to cell cycle checkpoint, and apoptosis were measured. Significantly increased DNA damages, decreased cells in the G1 phase, arrested cells at G2/M phase, decreased DNA repair protein XRCC1 foci formation, and enhanced cell apoptosis were observed in laryngeal cell lines treated by sodium glycididazole combined with irradiation compared with the irradiation alone. The combined treatment downregulated the protein expressions of ataxia–telangiectasia mutated (ATM), p-ATM, CHK2, and P53 but upregulated the protein expressions of MDM2 and Cdk2. This study indicates that sodium glycididazole enhances the radiosensitivity of laryngeal cancer cells through downregulation of ATM signaling pathway in vitro and in vivo.


Sodium glycididazole Laryngeal cancer Radiation-enhancing effect ATM signaling pathway 



This work was supported by the National Natural Science Foundation of China (No. 81201803) and by a grant from Luye Pharma Group Ltd. (Shanghai, China).

Compliance with ethical standard

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yue-Can Zeng
    • 1
  • Rui Xing
    • 1
  • Jing Zeng
    • 2
  • Ming Xue
    • 3
  • Feng Chi
    • 1
  • Yan Xin
    • 4
  • Guo-Liang Fan
    • 5
  • Hong-Mei Wang
    • 6
  • Qiong-Yu Duan
    • 1
  • Yu-Nan Sun
    • 1
  • Nan Niu
    • 1
  • Rong Wu
    • 1
  1. 1.Department of Medical Oncology, Cancer CenterShengjing Hospital of China Medical UniversityShenyangChina
  2. 2.Department of Radiation OncologyUniversity of Washington School of MedicineSeattleUSA
  3. 3.Department of Obstetrics and GynecologyShengjing Hospital of China Medical UniversityShenyangChina
  4. 4.Cancer InstituteNo.1 Hospital of China Medical UniversityShenyangChina
  5. 5.Department of OtorhinolaryngologyHarbin First HospitalHarbinChina
  6. 6.Department of Radiation OncologyNanfang Hospital of Southern Medical UniversityGuangzhouChina

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