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Tumor Biology

, Volume 37, Issue 3, pp 3897–3903 | Cite as

Apoptotic effects of salinomycin on human ovarian cancer cell line (OVCAR-3)

  • Fuat Kaplan
  • Fulya Teksen
Original Article

Abstract

In this study, we studied the apoptotic and cytotoxic effects of salinomycin on human ovarian cancer cell line (OVCAR-3) as salinomycin is known as a selectively cancer stem cell killer agent. We used immortal human ovarian epithelial cell line (IHOEC) as control group. Ovarian cancer cells and ovarian epithelial cells were treated by different concentrations of salinomycin such as 0.1, 1, and 40 μM and incubated for 24, 48, and 72 h. Dimethylthiazol (MTT) cell viability assay was performed to determine cell viability and toxicity. On the other hand, the expression levels of some of the apoptosis-related genes, namely anti-apoptotic Bcl-2, apoptotic Bax, and Caspase-3 were determined by quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, Caspase-3 protein level was also determined. As a result, we concluded that incubation of human OVCAR-3 by 0.1 μM concentration of salinomycin for 24 h killed 40 % of the cancer cells by activating apoptosis but had no effect on normal cells. The apoptotic Bax gene expression was upregulated but anti-apoptotic Bcl-2 gene expression was downregulated. Active Caspase-3 protein level was increased significantly (p < 0.05).

Keywords

Ovarian cancer Apoptosis Salinomycin Cell viability 

Notes

Acknowledgments

This Study was funded by the Ankara University BAP Project No: 13B3330001 and accepted as Master Thesis, by The Institute of Health Sciences.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Health Science Institute, BiochemistryHacettepe UniversityAnkaraTurkey
  2. 2.Medical Biology Department, Faculty of MedicineAnkara UniversityAnkaraTurkey

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