Tumor Biology

, Volume 37, Issue 3, pp 3969–3978 | Cite as

Do circulating long non-coding RNAs (lncRNAs) (LincRNA-p21, GAS 5, HOTAIR) predict the treatment response in patients with head and neck cancer treated with chemoradiotherapy?

  • Merdan Fayda
  • Mustafa Isin
  • Makbule Tambas
  • Murat Guveli
  • Rasim Meral
  • Musa Altun
  • Dilek Sahin
  • Gozde Ozkan
  • Yasemin Sanli
  • Husniye Isin
  • Emre Ozgur
  • Ugur Gezer
Original Article


Long non-coding RNAs (lncRNAs) have been shown to be aberrantly expressed in head and neck cancer (HNC). The aim of the present study was to evaluate plasma levels of three lncRNA molecules (lincRNA-p21, GAS5, and HOTAIR) in the treatment response in HNC patients treated with radical chemoradiotherapy (CRT). Forty-one patients with HNC were enrolled in the study. Most of the patients had nasopharyngeal carcinoma (n = 27, 65.9 %) and locally advanced disease. Blood was drawn at baseline and treatment evaluation 4.5 months after therapy. lncRNAs in plasma were measured by semiquantitative PCR. Treatment response was evaluated according to clinical examination, RECIST and PERCIST criteria based on magnetic resonance imaging (MRI), and positron emission tomography with computed tomography (PET/CT) findings. Complete response (CR) rates were 73.2, 36.6, and 50 % for clinical investigation, PET/CT-, or MRI-based response evaluation, respectively. Predictive value of lncRNAs was investigated in patients with CR vs. those with partial response (PR)/progressive disease (PD). We found that post-treatment GAS5 levels in patients with PR/PD were significantly higher compared with patients with CR based on clinical investigation (p = 0.01). Receiver operator characteristic (ROC) analysis showed that at a cutoff value of 0.3 of GAS5, sensitivity and specificity for clinical tumor response were 82 and 77 %, respectively. Interestingly, pretreatment GAS5 levels were significantly increased in patients with PR/PD compared to those with CR upon MRI-based response evaluation (p = 0.042). In contrast to GAS5, pretreatment or post-treatment lincRNA-p21 and HOTAIR levels were not informative for treatment response. Our results suggest that circulating GAS5 could be a biomarker in predicting treatment response in HNC patients.


Circulating long non-coding RNAs (lncRNAs) GAS5 LincRNA-p21 HOTAIR Head and neck Treatment response predictive value 



We would like to thank Atilla Bozdogan for performing the statistical analysis.

Compliance with ethical standard

Statement of human rights

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.

Conflicts of interest



The study was funded by Istanbul University Scientific Research Projects Coordination Unit (no: 28278).


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Merdan Fayda
    • 1
  • Mustafa Isin
    • 2
  • Makbule Tambas
    • 1
  • Murat Guveli
    • 1
  • Rasim Meral
    • 1
  • Musa Altun
    • 1
  • Dilek Sahin
    • 3
  • Gozde Ozkan
    • 4
  • Yasemin Sanli
    • 4
  • Husniye Isin
    • 2
  • Emre Ozgur
    • 2
  • Ugur Gezer
    • 2
  1. 1.Department of Radiation Oncology, Institute of OncologyIstanbul UniversityIstanbulTurkey
  2. 2.Department of Basic Oncology, Institute of OncologyIstanbul UniversityIstanbulTurkey
  3. 3.Oncological Radiology Unit, Institute of OncologyIstanbul UniversityIstanbulTurkey
  4. 4.Department of Nuclear Medicine, Istanbul Faculty of MedicineIstanbul UniversityIstanbulTurkey

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