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Tumor Biology

, Volume 37, Issue 5, pp 6501–6510 | Cite as

FAM172A is a tumor suppressor in colorectal carcinoma

Original Article

Abstract

The present study was designed to elucidate the regulatory role of a novel protein FAM172A in carcinogenesis of colorectal carcinoma (CRC). Investigation of clinical samples using Western blotting showed that expression of FAM172A is significantly lower in cancerous tissues than in adjacent tissues. Furthermore, we constructed in vitro model for continuous overexpression and silencing of FAM172A with a retroviral vector system. FAM172A suppressed the proliferative and invasive potentials of LOVO cells as shown in MTT test, transwell migration assay, wound healing assay, 3D-culture morphologic study, and xenograft experiment. RT-PCR and Western blotting showed that FAM172A overexpression inhibited expressions of Cyclin D1, CDK2, MMP-2, MMP-9, PERK, elF2α, ATF6, XBP1, and GRP78, while FAM172A silencing induced their expressions. FAM172A might regulate ERS through PERK-elF2α, ATF6-XBP1-GRP78 signal pathway. The results implicated that FAM172A functioned as a tumor suppressor in colorectal carcinoma.

Keywords

FAM172A Tumor suppressor Colorectal carcinoma 

Notes

Compliance with ethical standards

The protocol used in this study had been reviewed and approved by Medical Ethics Committee of Zhujiang Hospital, Southern Medical University. All participating patients were formally informed for the purpose of using their samples in this study, and a letter of consent had been signed by every patient participated.

Conflict of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of General Surgery, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  2. 2.Department of Ophthalmology, Zhujiang HospitalSouthern Medical UniversityGuangzhouChina
  3. 3.Department of Vascular Surgery, Nanfang HospitalSouthern Medical UniversityGuangzhouChina

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