Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study
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Metabolites are the end products of cellular regulatory processes. Squamous cervical cancer (SCC) can alter the level of certain small molecular metabolite in plasma through modulating gene expression. In this study, we identified two metabolites, phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), which are significantly down- and upregulated in plasma of SCC as compared to uterine fibroid (UF) patients via ultra-performance liquid chromatographic-mass spectrometry (UPLC-MS). In external prospective cohort, our assay has a sensitivity of 93.2 %, a specificity of 91.3 %, and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.972. The level of LPC is significantly higher in SCC than in UF patients. An opposite result was observed in PC level. Our findings suggest that the PC and lysoPC could be used as novel biomarkers to facilitate SCC diagnosis.
KeywordsMetabonomics Cervical cancer Phosphatidylcholine Lysophosphatidylcholine Biomarker
The study was supported by a grant from the National Natural Science Foundation of China (NSFC 81172453) and the Tumor Hospital of Harbin Medical University Starting Foundation (JJZ2011-08), and the present research was supported by the National Natural Science Foundation of China (No. 81274131), 2011 Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education, a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Project Program of the State Key Laboratory of Natural Medicines, China Pharmaceutical University (No. JKGZ201107).
Compliance with ethical standards
Procedures used in this study were approved by the ethics committee of the Tumor Hospital of Harbin Medical University.
Consent to participate
All subjects signed the consent form and agreed to have their plasma samples used for scientific purposes.
Conflicts of interest
- 3.Welthagen W, Shellie R, Ristow M, Spranger J, Zimmermann R, Fiehn O. Comprehensive two dimensional gas chromatography-time of flight mass spectrometry, GCxGC-TOF for high resolution metabolomics: biomarker discovery on spleen tissue extracts of obese NZO compared to lean C57BL/6 mice. Metabolomics. 2005;1:57–65.CrossRefGoogle Scholar
- 12.Yin M, Zhao F, Lou G, Zhang H, Sun M, Li C, et al. The long-term efficacy of neoadjuvant chemotherapy followed by radical hysterectomy compared with radical surgery alone or concurrent chemoradiotherapy on locally advanced-stage cervical cancer. Int J Gynecol Cancer. 2011;21(1):92–9.PubMedGoogle Scholar