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Tumor Biology

, Volume 37, Issue 4, pp 5485–5492 | Cite as

Identification of phosphatidylcholine and lysophosphatidylcholine as novel biomarkers for cervical cancers in a prospective cohort study

  • Ming-zhu Yin
  • Shu Tan
  • Xia Li
  • Yan Hou
  • Guosheng Cao
  • Kang Li
  • Junping Kou
  • Ge Lou
Original Article

Abstract

Metabolites are the end products of cellular regulatory processes. Squamous cervical cancer (SCC) can alter the level of certain small molecular metabolite in plasma through modulating gene expression. In this study, we identified two metabolites, phosphatidylcholine (PC) and lysophosphatidylcholine (LPC), which are significantly down- and upregulated in plasma of SCC as compared to uterine fibroid (UF) patients via ultra-performance liquid chromatographic-mass spectrometry (UPLC-MS). In external prospective cohort, our assay has a sensitivity of 93.2 %, a specificity of 91.3 %, and an area under the receiver operating characteristic (ROC) curve (AUC) of 0.972. The level of LPC is significantly higher in SCC than in UF patients. An opposite result was observed in PC level. Our findings suggest that the PC and lysoPC could be used as novel biomarkers to facilitate SCC diagnosis.

Keywords

Metabonomics Cervical cancer Phosphatidylcholine Lysophosphatidylcholine Biomarker 

Notes

Acknowledgments

The study was supported by a grant from the National Natural Science Foundation of China (NSFC 81172453) and the Tumor Hospital of Harbin Medical University Starting Foundation (JJZ2011-08), and the present research was supported by the National Natural Science Foundation of China (No. 81274131), 2011 Program for Excellent Scientific and Technological Innovation Team of Jiangsu Higher Education, a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, and the Project Program of the State Key Laboratory of Natural Medicines, China Pharmaceutical University (No. JKGZ201107).

Compliance with ethical standards

Procedures used in this study were approved by the ethics committee of the Tumor Hospital of Harbin Medical University.

Consent to participate

All subjects signed the consent form and agreed to have their plasma samples used for scientific purposes.

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.State Key Laboratory of Natural Products, Jiangsu Key Laboratory of TCM Evaluation and Translational Research Department of Complex Prescription of TCMChina Pharmaceutical UniversityNanjingChina
  2. 2.Department of Gynecology OncologyThe Tumor Affiliated Hospital of Harbin Medical UniversityHarbinChina
  3. 3.Department of PathologyYale School of MedicineNew HavenUSA
  4. 4.Department of Epidemiology and BiostatisticsHarbin Medical UniversityHarbinChina

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