Tumor Biology

, Volume 37, Issue 2, pp 2737–2748 | Cite as

A long non-coding RNA contributes to doxorubicin resistance of osteosarcoma

  • Chun-Lin Zhang
  • Kun-Peng Zhu
  • Guo-Qi Shen
  • Zhong-Sheng Zhu
Original Article

Abstract

Long non-coding RNAs (lncRNAs) are emerging in molecular biology as crucial regulators of cancer. Although the aberrant expression of lncRNAs has been observed in osteosarcoma (OS), the molecular mechanisms underlying lncRNAs in doxorubicin resistance of OS still unknown. In the current study, we investigated a novel lncRNA, termed ODRUL (osteosarcoma doxorubicin-resistance related up-regulated lncRNA), and evaluated its role in the occurrence of doxorubicin resistance in OS. LncRNA microarray revealed that lncRNA ODRUL was the most up-regulated expressed in the doxorubicin-resistant OS cell line. Quantitative real-time PCR (qRT-PCR) confirmed that lncRNA ODRUL was higher in different doxorubicin-resistant OS cell lines and lower in different doxorubicin-sensitive OS cell lines. Moreover, we showed that lncRNA ODRUL was increased in specimens of OS patients with a poor chemoresponse and lung metastasis. We further demonstrated that lncRNA ODRUL inhibition could inhibit OS cell proliferation, migration, and partly reversed doxorubicin resistance in vitro. In addition, we found that the expression of classical drug resistance-related ATP-binding cassette, subfamily B, member 1 (ABCB1) gene was decreased after the lncRNA ODRUL knockdown. Thus, we concluded that lncRNA ODRUL may act as a pro-doxorubicin-resistant molecule through inducing the expression of the classical multidrug resistance-related ABCB1 gene in osteosarcoma cells .These findings may provide a novel target for reversing doxorubicin resistance in OS.

Keywords

LncRNA ODRUL Osteosarcoma Doxorubicin resistance 

Abbreviations

LncRNA

Long non-coding RNA

OS

Osteosarcoma

ODRUL

Osteosarcoma doxorubicin resistance-related up-regulated lncRNA

Notes

Acknowledgments

This project was supported by a Grant from the National Natural Science Foundation of China (NSFC No. 81572630), Shanghai Pujiang Program of Shanghai Science and Technology Commission (NO.13PJD023) and Shanghai Jiaotong University Medical-Engineering Cross Research Fund (NO.YG2012MS49).

Competing interests

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Chun-Lin Zhang
    • 1
  • Kun-Peng Zhu
    • 2
  • Guo-Qi Shen
    • 3
  • Zhong-Sheng Zhu
    • 1
  1. 1.Department of Orthopaedic SurgeryShanghai Tenth People’s Hospital Affiliated to Tongji UniversityShanghaiPeople’s Republic of China
  2. 2.Department of Orthopaedic SurgeryShanghai Sixth People’s Hospital Affiliated to Shanghai Jiaotong UniversityShanghaiPeople’s Republic of China
  3. 3.Department of Orthopaedic Surgery, Shanghai Sixth People’s HospitalSoochow UniversityShanghaiPeople’s Republic of China

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