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Tumor Biology

, Volume 37, Issue 3, pp 3757–3764 | Cite as

ROCK inhibitor Y-27632 inhibits the growth, migration, and invasion of Tca8113 and CAL-27 cells in tongue squamous cell carcinoma

  • Zhi-Ming Wang
  • Dong-Sheng Yang
  • Jie Liu
  • Hong-Bo Liu
  • Ming Ye
  • Yu-Fei Zhang
Original Article

Abstract

The objective of this study is to determine the effects of Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor Y-27632 on the growth, invasion, and migration of Tca8113 and CAL-27 cells in tongue squamous cell carcinoma (TSCC). The methods of the study are as follows: After being routinely cultured for 24 h, Tca8113 and CAL-27 cells were treated with Y-27632 solution. The morphological change of Y-27632-treated cells was observed under an optical microscope and an inverted microscope; MTT assay was performed to measure the optical density (OD) of cells and calculate cell growth inhibition rate; the change of apoptosis was detected by AnnexinV-FITC/PI assay; cell invasion and migration were measured by Transwell assay. The results were as follows: (1) With increasing concentration of Y-27632, cell morphology changed and cell apoptosis appeared; (2) MTT assay showed that inhibition effect of Y-27632 on Tca8113 and CAL-27 cells was enhanced with increasing concentrations and time (all P < 0.01); (3) Apoptosis showed that, compared with controls, the number of apoptosis cells in experimental groups was significantly increased (all P < 0.01). Apoptosis rate was elevated with increasing concentrations of Y-27632; (4) Transwell assay showed, after a treatment with Y-27632, the number of migrated and invaded Tca8113 and CAL-27 cells in each group was statistically different (all P < 0.01); compared with controls, the number of migrated cell in groups treated with Y-27632 was decreased and less Tca8113 and CAL-27 cells in experimental groups passed through polycarbonate membrane (all P < 0.05). The study concludes that Y-27632 can inhibit the growth, invasion, and migration of Tca8113 and CAL-27 cells, suggesting that Y-27632 may be therapeutically useful in TSCC.

Keywords

ROCK inhibitor Y-27632 Tca8113 CAL-27 Tongue squamous cell carcinoma Cell growth Cell invasion Cell migration 

Notes

Acknowledgements

This study was supported by the Liaoning Science and Technology Project (No. 2012225100) and Natural Science Foundation of Liaoning Province (No. 20102286).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Zhi-Ming Wang
    • 1
  • Dong-Sheng Yang
    • 1
  • Jie Liu
    • 2
  • Hong-Bo Liu
    • 3
  • Ming Ye
    • 1
  • Yu-Fei Zhang
    • 1
  1. 1.Department of Oral and Maxillofacial SurgeryShengjing Hospital of China Medical UniversityShenyangChina
  2. 2.Experimental Technology Center of China Medical UniversityShenyangChina
  3. 3.Department of Statistics, School of Public HealthChina Medical UniversityShenyangChina

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