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Tumor Biology

, Volume 37, Issue 2, pp 2757–2764 | Cite as

Detection of DNA repair protein in colorectal cancer of patients up to 50 years old can increase the identification of Lynch syndrome?

  • Demétrius Eduardo Germini
  • Ana Maria Amaral Antônio Mader
  • Luiz Guilherme Lisboa Gomes
  • Thérèse Rachel Teodoro
  • Maria Isete Fares Franco
  • Jaques Waisberg
Original Article

Abstract

The aim of this study was to compare the results of protein level of the DNA mismatch repair genes with the clinical diagnosis of Lynch syndrome according to the Amsterdam II criteria in patients 50 years and younger who underwent surgery for colorectal cancer. The subjects of analysis were 48 patients 50 years old and younger. Immunohistochemistry assays were performed to detect proteins from the DNA mismatch repair genes. Clinicopathological data and Amsterdam II criteria for the diagnosis of hereditary nonpolyposis colorectal cancer were obtained by analyzing medical records. Two (4 %) patients satisfied the Amsterdam II criteria for Lynch syndrome, and both presented levels of all of the studied mismatch repair proteins. A total of 13 (27 %) patients exhibited the absence of protein levels of the studied mismatch repair genes. None of these patients were considered suspicious for Lynch syndrome according to the Amsterdam II criteria. Screening for the level of proteins of the mismatch repair system in all colorectal cancer patients 50 years and younger can increase the identification of patients with suspicion of Lynch syndrome.

Keywords

Colorectal neoplasms Hereditary nonpolyposis Lynch syndrome DNA mismatch repair MutS DNA mismatch-binding protein Immunohistochemistry 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Demétrius Eduardo Germini
    • 1
  • Ana Maria Amaral Antônio Mader
    • 2
  • Luiz Guilherme Lisboa Gomes
    • 3
  • Thérèse Rachel Teodoro
    • 4
  • Maria Isete Fares Franco
    • 5
  • Jaques Waisberg
    • 3
  1. 1.Department of SurgerySão Paulo State Civil Servant HospitalSão PauloBrazil
  2. 2.Department of PathologyABC Medical SchoolSanto AndréBrazil
  3. 3.Department of SurgeryABC Medical SchoolSanto AndréBrazil
  4. 4.Department of MorphologyABC Medical SchoolSanto AndréBrazil
  5. 5.Department of PathologySão Paulo State Civil Servant HospitalSão PauloBrazil

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