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Tumor Biology

, Volume 37, Issue 2, pp 2547–2553 | Cite as

Autoantibody response to Sui1 and its tissue-specific expression in hepatocellular carcinoma

  • Jian-Wei Zhou
  • Yuan Li
  • Li-Xia Yue
  • Cheng-Lin Luo
  • Yao Chen
  • Jian-Ying Zhang
Original Article

Abstract

To investigate the immunogenicity of Homo sapiens putative translation initiation factor (Sui1) in hepatocellular carcinoma (HCC), enzyme-linked immunosorbent assay (ELISA) and Western blot were utilized to assess autoantibody responses to Sui1 in sera from HCC patients and healthy individuals. Indirect immunofluorescence (IIF) assay with cancer cells and immunohistochemistry (IHC) study with tissue array slides were performed to examine Sui1 expression profile in cancer cells and tissues. The data confirmed that the frequency of autoantibody to Sui1 in sera of HCC patients was 15.5 % (16/103), which was remarkably higher than that in sera of liver cirrhosis (LC) patients (3.3 %, 1/30), chronic hepatitis (CH) patients (0 %, 0/29), and normal human serum (NHS) (0 %, 0/82) (p < 0.01). IHC study showed that the Sui1 expression in HCC tissues was 26.7 % (16/60). The expression of Sui1 had the trend of increasing along with the cancer grades but no statistical significance (p > 0.05). In immunodiagnosis of HCC, the sensitivity and specificity of the anti-Sui1 antibody were 15.5 and 99.3 %, respectively. If both anti-Sui1 and alpha fetal protein (AFP) were simultaneously utilized as detective markers, 66.7 % (30/45) of HCC patients could be correctly distinguished. The results suggested that anti-Sui1 could be utilized as a supplementary serological marker for the detection of HCC and Sui1 might be associated to HCC carcinogenesis.

Keywords

Sui1 Tumor-associated antigen Hepatocellular carcinoma Immunoreactivity Autoantibody 

Notes

Acknowledgments

This work was supported by a grant (SC1CA166016) from the National Institutes of Health (NIH). We also thank the Border Biological Research Center (BBRC) Core Facilities at The University of Texas at El Paso (UTEP) for their support, which were funded by a NIH grant (5G12MD007592).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Jian-Wei Zhou
    • 1
    • 2
  • Yuan Li
    • 2
    • 3
  • Li-Xia Yue
    • 2
    • 4
  • Cheng-Lin Luo
    • 2
  • Yao Chen
    • 2
  • Jian-Ying Zhang
    • 2
  1. 1.Department of OncologyPeople’s Hospital of Zhengzhou University (Henan Provincial People’s Hospital)ZhengzhouChina
  2. 2.Department of Biological SciencesThe University of Texas at El PasoEl PasoUSA
  3. 3.Department of StomatologyZhengzhou First People’s HospitalZhengzhouChina
  4. 4.Surgical DepartmentPeople’s Hospital of Zhengzhou University (Henan Provincial People’s Hospital)ZhengzhouChina

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