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Tumor Biology

, Volume 37, Issue 3, pp 3697–3704 | Cite as

Radiofrequency ablation-increased CXCL10 is associated with earlier recurrence of hepatocellular carcinoma by promoting stemness

  • Yabo Ouyang
  • Kai Liu
  • Meijun Hao
  • Rongling Zheng
  • Chunmiao Zhang
  • Yanning Wu
  • Xiaofeng Zhang
  • Ning Li
  • Jiasheng Zheng
  • Dexi Chen
Original Article

Abstract

Radiofrequency ablation (RFA) represents a valuable choice in hepatocellular carcinoma (HCC); however, local recurrence of HCC is common after RFA. Here, 20 primary HCC patients treated by RFA were enrolled. Before (termed 0d) and after RFA treatment for 1 and 7 days (termed 1d and 7d, respectively), plasma and noncancerous tissue were collected. ELISA assay showed that plasma C-X-C motif chemokine 10 (CXCL10) was increased in ten patients (type I patients) but decreased in the other 10 patients (type II patients). The mean interval for HCC recurrence in type I patients was less than the mean interval in type II patients. Interestingly, a significant negative correlation between interval for HCC recurrence and fold change of plasma CXCL10 (1d/0d or 7d/0d) was identified, suggesting that RFA-induced CXCL10 is associated with earlier HCC recurrence. Immunofluorescence assay showed that the receptor of CXCL10, chemokine (C-X-C motif) receptor 3 (CXCR3), was significantly increased in type I, but not type II, patients after RFA. In vitro assay demonstrated that CXCL10 stimulus increased the rate of CD133+ cancer stem cells (CSCs) in HepG2 cells by binding to CXCR3 and then inducing c-Myc expression. Many studies have reported that induction of CD133+ CSCs contributes to HCC recurrence. Thus, CXCL10-increased CD133+ CSCs by activating CXCR3/c-Myc pathway might accelerate HCC recurrence after RFA. These data might have potential implications for HCC therapy.

Keywords

CXCL10 CXCR3 Hepatocellular carcinoma Radiofrequency ablation Cancer stem cell 

Abbreviations

CSCs

Cancer stem cells

CXCL10

C-X-C motif chemokine 10

CXCR3

Chemokine (C-X-C motif) receptor 3

HCC

Hepatocellular carcinoma

MSCs

Mesenchymal stem cells

RFA

Radiofrequency ablation

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (812111545), the Twelfth Key Science and Technology Five-Year Plan of China (2012ZX10001-002, 2012ZX10001-003, and 2012ZX10001-004), the National Key Technology Support Program of China (2012BAI15B08), the Capital Health Research and Development Special (2014-1-1151), and the Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality (IDHT20150502).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yabo Ouyang
    • 1
    • 2
  • Kai Liu
    • 1
    • 2
  • Meijun Hao
    • 1
  • Rongling Zheng
    • 1
    • 2
  • Chunmiao Zhang
    • 1
    • 2
  • Yanning Wu
    • 1
    • 2
  • Xiaofeng Zhang
    • 1
    • 2
  • Ning Li
    • 1
    • 2
  • Jiasheng Zheng
    • 1
  • Dexi Chen
    • 1
    • 2
  1. 1.Beijing You’an HospitalCapital Medical UniversityBeijingChina
  2. 2.Beijing Institute of HepatologyBeijingChina

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