Tumor Biology

, Volume 37, Issue 2, pp 2575–2586 | Cite as

Overexpression and biological function of TMEM48 in non-small cell lung carcinoma

  • Wenliang Qiao
  • Yudong Han
  • Wei Jin
  • Mi Tian
  • Pei Chen
  • Jie Min
  • Haiyang Hu
  • Binbin Xu
  • Wenzhuo Zhu
  • Liwen Xiong
  • Qiang Lin
Original Article

Abstract

Transmembrane protein 48 (TMEM48), localized to nuclear pore complexes (NPCs), has been reported crucial for NPC assembly. Alterations in NPC members have been reported in several malignancies. The present study was aimed to elucidate the expression and biological function of TMEM48 in non-small cell lung carcinoma (NSCLC). Here, TMEM48 expression level was higher in NSCLC tissues than that in the adjacent normal tissues. Moreover, higher TMEM48 expression was correlated with a more advanced tumor stage, lymph node metastasis, bigger tumor size tumor stage, and shorter survival time. Knockdown of TMEM48 in NSCLC cell lines, A549 and H1299, inhibited cell proliferation and significantly increased cells population in G1 phase. Gene set enrichment analysis (GSEA) showed that cell cycle pathway was correlative with the TMEM48 expression. Additionally, real-time PCR and western blot analysis revealed that several cell cycle and DNA replication genes, including Cyclin B1, CDK1, CDC6, PCNA, and RCF4, were reduced after TMEM48 knockdown. Additionally, inhibition of TMEM48 in NSCLC cells significantly stimulated cell apoptosis, while notably repressed cell adhesion, migration, invasion, and tumorigenicity in nude mice. Our data provide insight into the biological relevance of TMEM48 in NSCLC progression and highlight its usefulness as a prognostic factor and potential therapeutic target in NSCLC.

Keywords

TMEM48 NSCLC Proliferation Cell cycle Metastasis 

Notes

Acknowledgments

This work was supported by grants from National Natural Science Foundation of China (81372521). We would like to also thank JRDUN Biotech. (Shanghai, China) for their technical assistance on bioinformatics analysis.

Author contributions

Conceived and designed the experiments: LWX QL. Performed the experiments: WLQ YDH BWS WJ MT PC. Analyzed the data: WLQ JM HYH. Contributed reagents/materials/analysis tools: BBX WZZ. Wrote the paper: WLQ QL.

Conflicts of interest

None

Supplementary material

13277_2015_4014_MOESM1_ESM.docx (1.1 mb)
Figure S1 (DOCX 1173 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Wenliang Qiao
    • 1
  • Yudong Han
    • 1
  • Wei Jin
    • 2
  • Mi Tian
    • 3
  • Pei Chen
    • 1
  • Jie Min
    • 4
  • Haiyang Hu
    • 1
  • Binbin Xu
    • 1
  • Wenzhuo Zhu
    • 1
  • Liwen Xiong
    • 5
  • Qiang Lin
    • 1
  1. 1.Department of Thoracic Surgery, Shanghai First People’s HospitalSchool of Medicine, Shanghai Jiao Tong UniversityShanghaiChina
  2. 2.Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of EducationSchool of Medicine, Shanghai Jiao Tong UniversityShanghaiChina
  3. 3.State Key Laboratory of Oncogenes and Related Genes, Shanghai Cancer Institute, Renji HospitalSchool of Medicine, Shanghai Jiao Tong UniversityShanghaiChina
  4. 4.Department of Cardiothoracic Surgery, Shanghai Changzheng HospitalThe Second Military Medical UniversityShanghaiChina
  5. 5.Department of Pulmonary Medicine, Shanghai Chest HospitalShanghai Jiao Tong UniversityShanghaiChina

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