Tumor Biology

, Volume 37, Issue 2, pp 2119–2126 | Cite as

Interleukin-16 polymorphisms as new promising biomarkers for risk of gastric cancer

  • Seyed Mohammad Hossein Kashfi
  • Faegheh Behboudi Farahbakhsh
  • Ehsan Nazemalhosseini Mojarad
  • Kazem Mashayekhi
  • Pedram Azimzadeh
  • Sara Romani
  • Shaghayegh Derakhshani
  • Habib Malekpour
  • Hamid Asadzadeh Aghdaei
  • Mohammad Reza Zali
Original Article


Gastric cancer (GC) is the second cause of cancer-related death worldwide. Interleukin (IL)-16 has a vital role in the development and homeostasis of the immune system. In the present study, we evaluated an exon variant rs4072111 C/T polymorphism and 3′ UTR variant rs1131445 C/T within the miRNA binding with gastric cancer susceptibility in Iranian population. Genomic DNA was isolated from peripheral blood samples according to phenol chloroform extraction. The genotypes of IL-16 polymorphisms rs1131445 T/C and rs4072111 T/C were determined by polymerase chain reaction-restriction fragment length polymorphism method. In this case control study, a total of 256 patients with gastric cancer (238 cases (92.9 %) non-cardia and 18 cases (7.1 %) cardia) and 300 healthy control subjects were evaluated. In the present study, we found a significant association between rs4072111 of IL-16 gene and risk of GC in Iranian population. Individuals with CT genotype showed a significant association with 1.79-fold increased risk of GC (P = 0.008; adjusted OR 1.792; 95 % CI 1.164–2.759). The significant association was also detected for T allele of rs4072111 and increased risk of GC (P < 0.001; adjusted OR 1.981; 95 % CI 1.354–2.900). We also observed statistically a significant relationship between rs1131445 of IL-16 CT genotype and GC risk. Carriers of IL-16 CT genotype compared with TT genotype had 1.44 times higher increased likelihood of GC (P = 0.048; adjusted OR 1.445; 95 % CI 1.003–2.084). After stratification according to gender, we observed that in rs1131445, CT and CC male carriers had a higher risk of GC than females (P = 0.08; adjusted OR 1.608; 95 % CI 0.945–2.737 and P = 0.08; adjusted OR 2.186; 95 % CI 0.897–5.325, respectively). We also observed that for male carriers with C allele in rs1131445, there was a 1.53-fold higher risk of GC risk than female subjects (P = 0.029; adjusted OR 1.53; 95 % CI 1.04.4–2.248). We found that the rs1131445 T/C and rs4072111 T/C variants of IL-16 were significantly associated with increased risk of GC in Iranian population.


Gastric cancer IL-16 Polymorphism 



We thank all patients and their families who supported us in this study.

Conflicts of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Seyed Mohammad Hossein Kashfi
    • 1
  • Faegheh Behboudi Farahbakhsh
    • 2
  • Ehsan Nazemalhosseini Mojarad
    • 1
  • Kazem Mashayekhi
    • 2
  • Pedram Azimzadeh
    • 2
  • Sara Romani
    • 1
  • Shaghayegh Derakhshani
    • 2
  • Habib Malekpour
    • 1
  • Hamid Asadzadeh Aghdaei
    • 2
  • Mohammad Reza Zali
    • 1
  1. 1.Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran
  2. 2.Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver DiseasesShahid Beheshti University of Medical SciencesTehranIran

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