Tumor Biology

, Volume 37, Issue 2, pp 2057–2065 | Cite as

Overexpression of long non-coding RNA HOTAIR leads to chemoresistance by activating the Wnt/β-catenin pathway in human ovarian cancer

Original Article

Abstract

Overexpression of HOTAIR (HOX antisense intergenic RNA) is significantly correlated with tumor progression and poor prognosis in human ovarian cancer. However, the underlying mechanisms are largely unknown. In the present study, we investigated the roles of HOTAIR in the initiation and chemoresistance of ovarian cancer. As our data show, HOTAIR overexpression promoted cell cycle progression (and thus cell proliferation) by activating the wnt/β-catenin signaling pathway. Likewise, knockdown of HOTAIR suppressed cell proliferation and arrested cell cycle at G1 phase via inhibition of wnt/β-catenin signaling. Moreover, the results of primary culture demonstrated that elevated HOTAIR expression correlated positively with chemoresistance in ovarian cancer. In vitro and in vivo, HOTAIR induced cellular resistance to cisplatin by activating the wnt/β-catenin pathway, which could be reversed by pre-treatment with the wnt/β-catenin inhibitor, XAV939. In conclusion, HOTAIR promotes the initiation and chemoresistance of ovarian cancer by activating wnt/β-catenin signaling, suggesting that HOTAIR might be a potent therapeutic target for ovarian cancer treatment.

Keywords

Ovarian cancer Long non-coding RNA HOTAIR Proliferation Cell cycle Chemoresistance 

Notes

Acknowledgments

Our study was supported by the Postdoctoral Science Foundation of China to Jing Li (2015M570635) and to Haifeng Qiu (2015M570634), and the National Natural Science Funds to Jing Li (81502262) and to Haifeng Qiu (81502261).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of Oncologythe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina
  2. 2.Department of Obstetrics and GynecologyShanghai Jiao Tong University Affiliated International Peace Maternity and Child Health HospitalShanghaiChina
  3. 3.Department of Gynecologic Oncologythe Affiliated Cancer Hospital of Zhengzhou University and Henan Provincal Cancer HospitalZhengzhouChina
  4. 4.Department of Obstetrics and Gynecologythe Affiliated Hospital of Jiangnan University and the 4th People’s Hospital of WuxiWuxiChina
  5. 5.Department of Obstetrics and Gynecologythe First Affiliated Hospital of Zhengzhou UniversityZhengzhouChina

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