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Tumor Biology

, Volume 37, Issue 2, pp 2709–2719 | Cite as

Fangchinoline suppresses the growth and invasion of human glioblastoma cells by inhibiting the kinase activity of Akt and Akt-mediated signaling cascades

  • Bingyu Guo
  • Peng Xie
  • Jingyuan Su
  • Tingting Zhang
  • Xiaoming Li
  • Guobiao Liang
Original Article

Abstract

Glioblastoma multiforme (GBM) is one of the most palindromic and malignant central nervous system neoplasms, and the current treatment is not effectual for GBM. Research of specific medicine for GBM is significant. Fangchinoline possesses a wide range of pharmacological activities and attracts more attentions due to its anti-tumor effects. In this study, two WHO grade IV human GBM cell lines (U87 MG and U118 MG) were exposed to fangchinoline, and we found that fangchinoline specifically inhibits the kinase activity of Akt and markedly suppresses the phosphorylation of Thr308 and Ser473 of Akt in human GBM cells. We also observed that fangchinoline inhibits tumor cell proliferation and invasiveness and induces apoptosis through suppressing the Akt-mediated signaling cascades, including Akt/p21, Akt/Bad, and Akt/matrix metalloproteinases (MMPs). These data demonstrated that fangchinoline exerts its anti-tumor effects in human glioblastoma cells, at least partly by inhibiting the kinase activity of Akt and suppressing Akt-mediated signaling cascades.

Keywords

Fangchinoline Glioblastoma multiforme Akt Phosphorylation Signaling cascade 

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Bingyu Guo
    • 1
  • Peng Xie
    • 1
  • Jingyuan Su
    • 1
  • Tingting Zhang
    • 1
  • Xiaoming Li
    • 1
  • Guobiao Liang
    • 1
  1. 1.Institute of Neurological Medicine, General Hospital of Shenyang Military CommandShenyangChina

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