Tumor Biology

, Volume 37, Issue 2, pp 1933–1940 | Cite as

Anti-proliferative and anti-invasive effects of ferulic acid in TT medullary thyroid cancer cells interacting with URG4/URGCP

  • Yavuz Dodurga
  • Canan Eroğlu
  • Mücahit Seçme
  • Levent Elmas
  • Çığır Biray Avcı
  • N. Lale Şatıroğlu-Tufan
Original Article

Abstract

Ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA), a common dietary plant phenolic compound, is abundant in fruits and vegetables. The aim of present study is to investigate the effects of FA on cell cycle, apoptosis, invasion, migration, and colony formation in the TT medullary thyroid cancer cell line. The effect of FA on cell viability was determined by using CellTiter-Glo assay. IC50 dose in the TT cells was detected as 150 μM. URG4/URGCP (upregulated gene-4/upregulator of cell proliferation) is a novel gene in full-length mRNA of 3.607 kb located on 7p13. It was determined that FA caused a decrease in the expression of novel gene URG4/URGCP, CCND1, CDK4, CDK6, BCL2, MMP2, and MMP9, a significant increase in the expression of p53, PARP, PUMA, NOXA, BAX, BID, CASP3, CASP9, and TIMP1 genes in TT human thyroid cancer cell line by using real-time PCR. It was found that FA in TT cells suppressed invasion, migration, and colony formation by using matrigel invasion chamber, wound healing, and colony formation assay, respectively. In conclusion, it is thought that FA indicates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, migration, and colony formation on TT cells.

Keywords

Ferulic acid Thyroid cancer TT cells URG4/URGCP 

Abbreviations

CCND1

CyclinD1

CTG

CellTiter-Glo

PARP

Poly(ADP-ribose) polymerase

URG4/URGCP

Upregulated gene-4/upregulator of cell proliferation

ECM

Extracellular matrix

MMP

Matrix metalloproteinase

MTC

Medullary thyroid cancer

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Yavuz Dodurga
    • 1
  • Canan Eroğlu
    • 2
  • Mücahit Seçme
    • 1
  • Levent Elmas
    • 1
  • Çığır Biray Avcı
    • 3
  • N. Lale Şatıroğlu-Tufan
    • 4
  1. 1.Department of Medical BiologyPamukkale University, School of MedicineDenizliTurkey
  2. 2.Department of Medical BiologyNecmettin Erbakan University, School of MedicineKonyaTurkey
  3. 3.Department of Medical BiologyEge University, School of MedicineİzmirTurkey
  4. 4.Department of Forensic MedicineAnkara University, School of MedicineAnkaraTurkey

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