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Anti-proliferative and anti-invasive effects of ferulic acid in TT medullary thyroid cancer cells interacting with URG4/URGCP

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Tumor Biology

Abstract

Ferulic acid (4-hydroxy-3-methoxycinnamic acid; FA), a common dietary plant phenolic compound, is abundant in fruits and vegetables. The aim of present study is to investigate the effects of FA on cell cycle, apoptosis, invasion, migration, and colony formation in the TT medullary thyroid cancer cell line. The effect of FA on cell viability was determined by using CellTiter-Glo assay. IC50 dose in the TT cells was detected as 150 μM. URG4/URGCP (upregulated gene-4/upregulator of cell proliferation) is a novel gene in full-length mRNA of 3.607 kb located on 7p13. It was determined that FA caused a decrease in the expression of novel gene URG4/URGCP, CCND1, CDK4, CDK6, BCL2, MMP2, and MMP9, a significant increase in the expression of p53, PARP, PUMA, NOXA, BAX, BID, CASP3, CASP9, and TIMP1 genes in TT human thyroid cancer cell line by using real-time PCR. It was found that FA in TT cells suppressed invasion, migration, and colony formation by using matrigel invasion chamber, wound healing, and colony formation assay, respectively. In conclusion, it is thought that FA indicates anticarcinogenesis activity by affecting cell cycle arrest, apoptosis, invasion, migration, and colony formation on TT cells.

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Abbreviations

CCND1:

CyclinD1

CTG:

CellTiter-Glo

PARP:

Poly(ADP-ribose) polymerase

URG4/URGCP :

Upregulated gene-4/upregulator of cell proliferation

ECM:

Extracellular matrix

MMP:

Matrix metalloproteinase

MTC:

Medullary thyroid cancer

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Author information

Authors and Affiliations

  1. Department of Medical Biology, Pamukkale University, School of Medicine, Denizli, Turkey

    Yavuz Dodurga, Mücahit Seçme & Levent Elmas

  2. Department of Medical Biology, Necmettin Erbakan University, School of Medicine, Konya, Turkey

    Canan Eroğlu

  3. Department of Medical Biology, Ege University, School of Medicine, İzmir, Turkey

    Çığır Biray Avcı

  4. Department of Forensic Medicine, Ankara University, School of Medicine, Ankara, Turkey

    N. Lale Şatıroğlu-Tufan

Authors
  1. Yavuz Dodurga
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  2. Canan Eroğlu
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  3. Mücahit Seçme
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  4. Levent Elmas
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  5. Çığır Biray Avcı
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  6. N. Lale Şatıroğlu-Tufan
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Correspondence to Yavuz Dodurga.

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Dodurga, Y., Eroğlu, C., Seçme, M. et al. Anti-proliferative and anti-invasive effects of ferulic acid in TT medullary thyroid cancer cells interacting with URG4/URGCP . Tumor Biol. 37, 1933–1940 (2016). https://doi.org/10.1007/s13277-015-3984-z

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  • DOI: https://doi.org/10.1007/s13277-015-3984-z

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