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Tumor Biology

, Volume 37, Issue 2, pp 1781–1788 | Cite as

Circulating levels of the miRNAs, miR-194, and miR-29b, as clinically useful biomarkers for colorectal cancer

  • Gholam Basati
  • Amirnader Emami Razavi
  • Iraj Pakzad
  • Fardin Ali Malayeri
Original Article

Abstract

The microRNAs (miRNAs), miR-194 and miR-29b, have been shown to downregulate in colorectal cancer (CRC) and may identify and classify CRC patients as compared with those in control subjects. In the current study, we aimed to explore whether the serum levels of the miRNAs could be potential biomarkers for diagnosis and prognosis of CRC. A quantitative reverse-transcription polymerase chain reaction (qRT-PCR) assay was utilized to determine and compare serum levels of miR-194 and miR-29b in 55 patients with CRC and 55 control subjects. The correlations between levels of the miRNAs and clinicopathological stages of cancer were analyzed in patients. Receiver operating characteristic (ROC) curve and survival analyses were carried out, respectively, to determine diagnostic and prognostic values of the miRNAs. Serum levels of miR-194 and miR-29b were found to be significantly lower in CRC patients than those in control subjects (P < 0.0001). Moreover, serum levels of the miRNAs in patients were inversely correlated with the advanced TNM stages (P = 0.01). ROC curve and survival analyses revealed that reduced levels of the miRNAs could serve as diagnostic and prognostic biomarkers for patients with CRC (P = 0.0001). Serum levels of miR-194 and miR-29b may serve as potential biomarkers for diagnosis and prognosis of CRC.

Keywords

Serum miR-194 miR-29b Colorectal cancer TNM staging qRT-PCR 

Notes

Acknowledgments

This study was funded by the Ilam University of Medical Sciences (grant number EC/92/H/136).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Gholam Basati
    • 1
  • Amirnader Emami Razavi
    • 2
  • Iraj Pakzad
    • 3
  • Fardin Ali Malayeri
    • 4
  1. 1.Department of Clinical Biochemistry, Faculty of Allied Medical SciencesIlam University of Medical ScienceIlamIran
  2. 2.Iran National Tumor Bank, Cancer InstituteTehran University of Medical SciencesTehranIran
  3. 3.Department of Microbiology, Faculty of MedicineIlam University of Medical ScienceIlamIran
  4. 4.Department of Clinical Biochemistry, Faculty of MedicineZabol University of Medical SciencesZabolIran

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