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Tumor Biology

, Volume 37, Issue 2, pp 1817–1824 | Cite as

Neo-epitopes on crotonaldehyde modified DNA preferably recognize circulating autoantibodies in cancer patients

  • Badar ul Islam
  • Parvez Ahmad
  • Gulam Rabbani
  • Kiran Dixit
  • Moinuddin
  • Shahid Ali Siddiqui
  • Asif Ali
Original Article

Abstract

DNA damage is one of the leading causes of various pathological conditions including carcinogenesis. Crotonaldehyde is a 4-carbon unsaturated bifunctional aldehyde which is found ubiquitously and produced both exogenously and endogenously. It reacts with deoxyguanosine and form adducts with DNA. These adducts were detected and found involved in tumor formation in rats treated with crotonaldehyde. In the present study, structural changes in DNA by crotonaldehyde were evaluated by Fourier transform infrared (FTIR) spectroscopy, differential scanning colorimetry (DSC), dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), and atomic force microscopy (AFM). Enhanced binding was observed in cancer autoantibodies with the DNA modified by crotonaldehyde than the native counterpart. Immunological studies revealed enhanced binding of cancer autoantibodies with crotonaldehyde modified DNA, compared to the native form. Furthermore, lymphocyte DNA isolated from cancer patients demonstrated considerable recognition of anti-Cro-DNA IgG as compared to the DNA from healthy individuals. Therefore, we suggest that crotonaldehyde modified DNA presents unique epitopes, that may trigger autoantibody induction in cancer patients.

Keywords

Human DNA Crotonaldehyde Crosslinking Neo-epitopes Cancer 

Notes

Acknowledgments

One of the authors B. U. I. is thankful to University Grant Commission-Maulana Azad National Fellowship (UGC-MANF) for financial support as Senior Research Fellow (2011-12-MANF-MUS-UTT-24). Assistance from the Institution (AMU) as well as infrastructural support from DST-FIST to the department is duly acknowledged. The authors are grateful to Inderchand Rajgarhia and Sons Pvt. (India) Ltd for providing mica sheets.

Funding

None.

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Badar ul Islam
    • 1
  • Parvez Ahmad
    • 1
  • Gulam Rabbani
    • 2
  • Kiran Dixit
    • 1
  • Moinuddin
    • 1
  • Shahid Ali Siddiqui
    • 3
  • Asif Ali
    • 1
  1. 1.Department of Biochemistry, J. N. Medical CollegeAligarh Muslim UniversityAligarhIndia
  2. 2.Interdisciplinary Biotechnology UnitAligarh Muslim UniversityAligarhIndia
  3. 3.Department of Radiotherapy, J. N. Medical CollegeAligarh Muslim UniversityAligarhIndia

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