Tumor Biology

, Volume 37, Issue 1, pp 1121–1129 | Cite as

Downregulated ECRG4 is associated with poor prognosis in renal cell cancer and is regulated by promoter DNA methylation

  • Liya Luo
  • Jianting Wu
  • Jun Xie
  • Lingling Xia
  • Xuemin Qian
  • Zhiming Cai
  • Zesong Li
Original Article


Esophageal cancer-related gene 4 (ECRG4) has been proposed as a putative tumor suppressor gene in several tumors. However, the role and regulation of ECRG4 in the pathogenesis of human renal cancer remain largely unknown. Our current study revealed that expression of ECRG4 is downregulated in renal cell lines and renal cancer tissues. ECRG4 expression was significantly associated with histological grade of tumors (p < 0.001), primary tumor stage (p = 0.017), and distant metastasis (p = 0.017). Low expression of ECRG4 was an independent prognostic indicator for survival of renal cancer patients. Silencing of ECRG4 expression in renal cell lines was associated with its promoter methylation. Moreover, ectopic expression of ECRG4 markedly inhibited cell proliferation and invasion in renal cancer cell lines. These results indicated that ECRG4 is frequently silenced by the methylation of promoter in renal cell cancers. ECRG4 may be a tumor suppressor in renal cancer and serve as a prognostic marker.


Renal cancer Prognosis ECRG4 Immunohistochemistry DNA methylation 



This work was supported by grants from the Young Scientists Fund of the National Natural Science Foundation of China (Grant No. 81201579), the National Key Scientific Program of China (Grant No. 2014CBA02005), and the National Natural Science Foundation of China (Grant No. 81272840, 81472584), Shenzhen Project of Science and Technology (Grant No. 20140416180149160, 201206133000192).

Conflicts of interest


Supplementary material

13277_2015_3913_MOESM1_ESM.doc (32 kb)
Supplementary Fig. 1 C2orf40 expression in Bittner renal cancer


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Liya Luo
    • 1
    • 2
  • Jianting Wu
    • 1
  • Jun Xie
    • 3
  • Lingling Xia
    • 1
    • 2
  • Xuemin Qian
    • 1
  • Zhiming Cai
    • 1
  • Zesong Li
    • 1
  1. 1.Shenzhen Key Laboratory of Genitourinary TumorShenzhen Second People’s Hospital, First Affiliated Hospital of Shenzhen UniversityShenzhenChina
  2. 2.Guangzhou Medical UniversityGuangzhouChina
  3. 3.Peking University Shenzhen HospitalShenzhenChina

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