Tumor Biology

, Volume 37, Issue 1, pp 999–1008 | Cite as

Diagnostic and prognostic significance of secretory clusterin expression in patients with hepatocellular carcinoma

  • Wenjie Zheng
  • Min Yao
  • Wenli Sai
  • Qi Qian
  • Liuhong Pan
  • Liwei Qiu
  • Jianfei Huang
  • Wei Wu
  • Dengfu Yao
Original Article

Abstract

The upregulation of secretory clusterin (sCLU) is associated with tumor progression by contributing to angiogenesis, chemo-resistance, cell survival, and metastasis. However, its diagnostic or prognostic values for hepatocellular carcinoma (HCC) still remain to be clarified. The average serum sCLU level analyzed by an enzyme-linked immunosorbent assay was significantly higher (P < 0.001) in HCC patients than that in any of cases with cirrhosis, chronic hepatitis, or healthy control. The area under receiver operating characteristic curve and diagnostic sensitivity were 0.75 and 74.7 % in sCLU, and 0.74 and 58.7 % in α-fetoprotein (AFP), respectively. The combining detections of sCLU and AFP rose up to 90.7 % for HCC diagnosis. In liver, sCLU by immunohistochemistry was significantly higher (P < 0.001) in the HCC (77.3 %) group than that in their para-cancerous group (33.3 %). Abnormal serum or tissue sCLU expression was closely associated with tumor–node–metastasis (TNM) classification of malignant tumors and lymph node metastasis, as an independent prognosis factor (hazard ratio, 2.287; 95 % confidence interval, 1.044–5.007; P = 0.039), and higher sCLU expression significantly correlated (χ 2 = 4.252, P = 0.039) with poor survival of HCC patients analyzed by multivariate Cox regression or Kaplan–Meier method, suggesting that abnormal sCLU expression associated with tumor progression could be a potential diagnostic and prognostic biomarker for HCC.

Keywords

Hepatocellular carcinoma Clusterin Diagnosis Prognosis 

Abbreviations

AFP

Alpha-fetoprotein

CLU

Clusterin

ELISA

Enzyme-linked immunosorbent assay

HBV

Hepatitis B virus

HCC

Hepatocellular carcinoma

HCV

Hepatitis C virus

IHC

Immunohistochemistry

OS

Overall survival

TMA

Tissue microarray

Notes

Acknowledgments

This work was supported by grants-in-aid from some Projects of the Chinese National Natural Science Foundation (81200634), the Nantong Society Development (HS2014078), the Jiangsu Health Projects (BL2012053, YY-028, K201102, and WSN-078), the Priority Academic Program Development of Jiangsu (PADA), and the International S&T Cooperation Program of China (2013DFA32150).

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Wenjie Zheng
    • 1
  • Min Yao
    • 2
  • Wenli Sai
    • 1
  • Qi Qian
    • 3
  • Liuhong Pan
    • 3
  • Liwei Qiu
    • 1
  • Jianfei Huang
    • 4
  • Wei Wu
    • 1
  • Dengfu Yao
    • 1
  1. 1.Research Center of Clinical MedicineAffiliated Hospital of Nantong UniversityNantongChina
  2. 2.Department of ImmunologyMedical School of Nantong UniversityNantongChina
  3. 3.Department of OncologyAffiliated Hospital of Nantong UniversityNantongChina
  4. 4.Department of PathologyAffiliated Hospital of Nantong UniversityNantongChina

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