Tumor Biology

, Volume 37, Issue 1, pp 829–835 | Cite as

Adaptive B cell responses in rituximab-treated diffuse large B cell lymphoma patients during complete remission

  • Zhanshan Cha
  • Chen Li
  • Yan Zang
  • Haihui Gu
  • Huijun Guo
  • Jinqi Li
  • Yuan Fang
  • Thomas F. Petersen
  • Jing Li
  • Richard O. Karas
  • Michele L. Hamilton
  • Baohua Qian
Original Article


Rituximab is a chimeric monoclonal antibody directed against the CD20 antigen. Treatment using rituximab in combination with chemotherapy has dramatically improved overall survival rate of diffuse large B cell lymphoma (DLBCL). Since rituximab can deplete both lymphoma B cells and normal B cells, how rituximab-treatment affects normal B cell function in DLBCL patients under remission is unclear. Here, we examined peripheral blood B cell composition and antigen-specific B cell responses in DLBCL patients in remission and observed reductions in the frequencies of total B cell as well as several major B cell subsets, including CD19+IgD+ naive B cells, CD19+IgDCD27+ memory B cells, and CD19loCD27hi plasmablasts. Moreover, tetanus toxin (TT)-specific B cell proliferation was reduced in DLBCL patients in remission. On the other hand, HA-specific IgG-secreting B cell responses could be stimulated by influenza vaccination in DLBCL patients in remission, demonstrating that the machinery for generating de novo adaptive B cell responses was functional in DLBCL patients in remission. Our results provided insights in normal B cell function in DLBCL patients in remission.


B cell Rituximab BLDCL Remission 


Conflict of interest



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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Zhanshan Cha
    • 1
  • Chen Li
    • 2
  • Yan Zang
    • 1
  • Haihui Gu
    • 1
  • Huijun Guo
    • 1
  • Jinqi Li
    • 1
  • Yuan Fang
    • 3
  • Thomas F. Petersen
    • 3
  • Jing Li
    • 4
  • Richard O. Karas
    • 5
  • Michele L. Hamilton
    • 6
  • Baohua Qian
    • 1
  1. 1.Department of TransfusionChanghai Hospital, Second Military Medical UniversityShanghaiChina
  2. 2.Department of Hepatobiliary SurgeryLinyi People’s HospitalLinyiChina
  3. 3.BGC Biotechnology Research CenterJinanChina
  4. 4.Department of HematologyShandong Affiliated HospitalShandongChina
  5. 5.School of MedicineWayne State UniversityDetroitUSA
  6. 6.Department of MedicineUniversity of MarylandBaltimoreUSA

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