Tumor Biology

, Volume 37, Issue 2, pp 2145–2152 | Cite as

Low-level expression of microRNA-375 predicts poor prognosis in hepatocellular carcinoma

  • Na Zhou
  • Jinhua Wu
  • Xiang Wang
  • Zhao Sun
  • Qin Han
  • Lin Zhao
Original Article


MicroRNAs are predicted to play fundamental roles in the tumorigenesis of hepatocellular carcinoma (HCC). MiR-375 is frequently downregulated in HCC and acts as a tumor suppressor by targeting multiple oncogenes. The objective of this study was to evaluate miR-375 expression and its relevance to the prognosis of HCC. MiR-375 expression was measured in cancerous tissues using qRT-PCR and dichotomized based on a median cutoff. The association between miR-375 expression and clinicopathological parameters and prognosis was subsequently determined. Expression levels of miR-375 were detected in a cohort of 38 HCC patients who underwent curative surgery. No significant correlations were observed between miR-375 expression and clinicopathological parameters, such as gender, age, performance status, preoperative serum AFP level, histological grade, HBV-DNA copy number, ascites, cirrhosis, tumor size, number of tumor nodules, and macrovascular invasion. However, miR-375 expression differs across CLIP scores significantly (p < 0.05). A trend toward poorer disease-free survival (DFS) was observed in patients with lower miR-375 expression compared to those with higher miR-375 expression (p = 0.307). Multivariate analysis demonstrated that low miR-375 expression was an independent prognostic predictor for progression (p = 0.032, risk ratio 3.273). Subgroup analysis revealed that low expression of miR-375 was significantly associated with adverse DFS in patients with poorly differentiated histology, higher serum AFP level (≥400 ng/ml), and advanced tumor stage (CLIP score 1∼3) (p = 0.017, 0.009, and 0.024, respectively). Our study demonstrates that miR-375 expression is significantly correlated with DFS and may be a potential prognostic biomarker of disease progression in HCC.


Hepatocellular carcinoma Invasion MicroRNA-375 



Hepatocellular carcinoma


Hepatitis B virus


Quantitative reverse transcription polymerase chain reaction




The Eastern Cooperative Oncology Group Performance Status


The Cancer of the Liver Italian Program


Transcatheter hepatic arterial chemoembolization


Disease-free survival


Overall survival



This work was supported by grants from the National Natural Science Foundation of China (No. 81101588).

Conflicts of interest


Authors’ contributions

Na Zhou: conceived the study and managed its design and coordination.

Jinhua Wu and Wang Xiang: analysis and interpretation of data.

Zhao Sun and Qin Han: have given final approval of the version to be published.

Lin Zhao: have made substantial contributions to conception and design.

Supplementary material

13277_2015_3841_MOESM1_ESM.doc (28 kb)
ESM 1 (DOC 28 kb)
13277_2015_3841_MOESM2_ESM.doc (28 kb)
ESM 2 (DOC 27 kb)
13277_2015_3841_MOESM3_ESM.doc (44 kb)
ESM 3 (DOC 44 kb)
13277_2015_3841_Fig4_ESM.jpg (26 kb)
Supplementary Fig. 1

The melting curves and amplification curves of real-time PCR of miR-375. (A) melting curves; (B) amplification curve (JPEG 26 kb)

13277_2015_3841_MOESM4_ESM.tif (1.9 mb)
High Resolution Image (TIFF 1947 kb)


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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Na Zhou
    • 1
  • Jinhua Wu
    • 2
  • Xiang Wang
    • 1
  • Zhao Sun
    • 1
  • Qin Han
    • 2
  • Lin Zhao
    • 1
  1. 1.Department of Oncology, Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingPeople’s Republic of China
  2. 2.Institute of Basic Medical Sciences Chinese Academy of Medical SciencesSchool of Basic Medicine Peking Union Medical CollegeBeijingPeople’s Republic of China

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