Tumor Biology

, Volume 37, Issue 2, pp 1909–1918 | Cite as

iTRAQ-based quantitative proteomic analysis of esophageal squamous cell carcinoma

  • Feiyan Deng
  • Keming Zhou
  • Qiaoxin Li
  • Dong Liu
  • Mengyan Li
  • Hui Wang
  • Wei Zhang
  • Yuqing Ma
Original Article


Esophageal squamous cell carcinoma (ESCC) is one of the most common cancers. In this study, our objective was to identify differentially regulated proteins in ESCC using isobaric tag for relative and absolute quantification (iTRAQ) technique and liquid chromatography–tandem mass spectrometry (LC–MS/MS). We compared the protein expression profiles of ESCC tumor tissues with the corresponding adjacent normal tissue from three patients. It was determined that 72 and 57 unique proteins were significantly up-regulated and down-regulated in all three samples. In addition, there were 431 significantly differentially regulated proteins having at least two biological samples. This subject found some of the differential proteins, such as prolyl 4-hydroxylase subunit alpha-1, prolyl 4-hydroxylase subunit alpha-2, and calponin-2, immunoglobulin superfamily containing leucine-rich repeat protein, and prolyl 3-hydroxylase1, which were few studies about them in ESCC. In order to determine the results, we performed another independent experiment. Our results indicated quantitative proteomics, as a robust discovery tool for the identification, differentially regulated proteins in cancers.


ESCC Proteomic analysis ITRAQ 2DE/MALDI-TOF MS 


Conflicts of interest



This study was supported by the grant from the National Natural Science Foundation of China, NO: 81260308, and the Open Subject about Xinjiang Major Diseases of China, NO: SKLIB-XJMDR-2014-12.

Supplementary material

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of PathologyFirst Affiliated Hospital, Xinjiang Medical UniversityUrumqiChina
  2. 2.Hypertension Center of the People’s Hospital of Xinjiang Uygur Autonomous RegionHypertension Institute of Xinjiang Uygur Autonomous RegionUrumuqiChina
  3. 3.Department of PathologyZhunDong production worker hospital of Petro China Xinjiang oilfield companyChangjiChina

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