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Tumor Biology

, Volume 37, Issue 1, pp 419–424 | Cite as

CCR7 regulates Twist to induce the epithelial-mesenchymal transition in pancreatic ductal adenocarcinoma

  • Kexin Li
  • Baofeng Xu
  • Guangying Xu
  • Rui Liu
Original Article

Abstract

As reported, the CC chemokine receptor 7 (CCR7) trigger a series of signaling cascades in the epithelial-mesenchymal transition (EMT) of some malignancies. Meanwhile, Twist promotes EMT in pancreatic ductal adenocarcinoma (PDAC) progression. Here, effects of Twist on CCR7-induced EMT in the PDAC were investigated in detail. The immunohistochemistry was used to detect the expression of Twist, and then, in vitro assays were applied. The expression rate of Twist was 72.0 % in PDAC samples and closely correlated with tumor-node-metastasis (TNM) stage and invasion. When PDAC cell line PANC1 was subjected to CCL19 stimulation, the expression of p-ERK, p-AKT, Twist, N-cadherin, MMP9, and α-smooth muscle actin (α-SMA) was induced, while the GSK1120212, BEZ235, and MK2206 prohibited the increase of Twist and EMT biomarkers. For another thing, the si-Twist treatment attenuated CCL19-stimulated EMT occurrence, migration, and invasion phenotypes of PANC1 cells. In conclusion, CCR7 pathway up-regulates Twist expression via ERK and PI3K/AKT signaling to manage the EMT of PDAC. Our work allows for clinical gene or protein-targeted regimen of PDAC patients in the near future.

Keywords

CCR7 Twist EMT PDAC 

Notes

Acknowledgments

This study was supported by grants to BFX from Foundation of Jilin Provincial Science & Technology Department [No. 201201044].

Conflicts of interest

None

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Department of EndocrinologyPeople’s Hospital of Jilin ProvinceChangchunChina
  2. 2.Department of NeurosurgeryFirst Hospital of Jilin UniversityChangchunChina
  3. 3.Changchun Emergency CenterChangchunChina
  4. 4.Department of EndocrinologyChina-Japan Union Hospital of Jilin UniversityChangchunChina

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