Tumor Biology

, Volume 37, Issue 1, pp 723–727 | Cite as

RETRACTED ARTICLE: Survivin mRNA-circulating tumor cells are associated with prostate cancer metastasis

  • Huansheng Wang
  • Mingshan Yang
  • Jian Xu
  • Benkui Zou
  • Qian Zhou
  • Jiasheng Bian
  • Xingwu Wang
Original Article

Abstract

Detection of circulating tumor cells (CTCs) has been made to develop reliable assays for early diagnosis of various cancers. Overexpression of survivin in cancer cells is strongly associated with tumor progression. Although upregulation of survivin is observed in various tumors, its expression profile in the peripheral blood of prostate cancer (PCa) patients has not yet been investigated. In this study, we validated the application of survivin as the tumor marker to detect CTC and assessed its utility for diagnosis of PCa distant metastasis. Immunohistochemistry and quantitative real-time PCR (QRT-PCR) were performed to confirm the levels of surviving expression in PCa tissues. In addition, CTC values in 3 mL of peripheral blood from PCa patients, benign prostate hyperplasia (BPH) patients, and normal controls were also measured by the survivin-targeted PCR. Our results showed that surviving was overexpressed in PCa tissues. The median levels of blood surviving mRNA of PCa patients, BPH patients, and normal controls were 5.67 (range from 0 to 12.46), 2.24 (range from 0 to 6.55), and 1.85 (range from 0 to 3.82), respectively. The levels of survivin are positively associated with PCa distant metastasis. Our results concluded that quantitation of CTCs through survivin-PCR could be a promising marker for diagnosis of PCa metastasis.

Keywords

Prostate cancer Circulating tumor cell Survivin mRNA Metastasis 

Notes

Acknowledgments

This paper was supported by the National Key Basic Research Development Plan (973 Plan) (2011CB504302).

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Huansheng Wang
    • 1
  • Mingshan Yang
    • 1
  • Jian Xu
    • 1
  • Benkui Zou
    • 1
  • Qian Zhou
    • 1
  • Jiasheng Bian
    • 1
  • Xingwu Wang
    • 2
  1. 1.Department of Urology SurgeryShandong Cancer HospitalJinanChina
  2. 2.Department of Central LaboratoryShandong Cancer HospitalJinanChina

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