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Tumor Biology

, Volume 37, Issue 1, pp 463–471 | Cite as

MiR-375 targets KLF4 and impacts the proliferation of colorectal carcinoma

  • Qiqi Mao
  • Tao Quan
  • Bin Luo
  • Xuefeng Guo
  • Lei Liu
  • Qinghui Zheng
Original Article

Abstract

MiR-375 has been identified as oncogenes or tumor suppressor genes which has the potential to the development and growth of cancers. However, the limited information concerning the expression and role of miR-375 in colorectal cancer (CRC) is available. In this work, we provide evidence for a function of miR-375 in the inhibition of CRC proliferation. Here, we showed that miR-375, down-modulated in human colorectal cancer tissues compared with normal human colon tissues, including several colorectal cancer cell lines. Subsequently, using the luciferase reporter assays, we found that the KLF4 untranslated region (3′UTR) carries the direct binding site of miR-375. In terms of function in vitro, CCK-8 assay, colony formation assay, and cell cycle assay demonstrated that the overexpression of miR-375 suppressed CRC cell proliferation. Inhibition of KLF4 performed similar effects with miR-375 overexpression on CRC cells, and overexpression of KLF4 could significantly reverse the tumor suppressive effects of miR-375 on CRC cells. Furthermore, we found overexpressed miR-375 effectively repressed tumor growth via KLF4 in xenograft animal experiment. Taken together, these results illustrated that miR-375 depresses proliferation of CRC through regulating 3′UTR of KLF4 mRNA, which might be a promising therapeutic target for treating colorectal cancers.

Keywords

MiR-375 KLF4 Colorectal cancer Proliferation 

Notes

Acknowledgments

None.

Conflicts of interest

None.

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  1. 1.Medical Scientific Research CentreGuangxi Medical UniversityNanningPeople’s Republic of China
  2. 2.Department of Gastrointestinal SurgeryFirst Affiliated Hospital of Guangxi Medical UniversityNanningPeople’s Republic of China
  3. 3.Department of Gastrointestinal SurgeryAffiliated Tumor Hospital of Guangxi Medical UniversityNanningPeople’s Republic of China

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