Tumor Biology

, Volume 37, Issue 1, pp 601–610 | Cite as

Myocyte enhancer factor 2D promotes tumorigenicity in malignant glioma cells

  • Youguang Zhao
  • Ying Li
  • Yuan Ma
  • Songtao Wang
  • Jingmin Cheng
  • Tao Yang
  • Zhiyong Sun
  • Yongqin Kuang
  • Haidong Huang
  • Kexia Fan
  • Jianwen Gu
Original Article

Abstract

The prognosis of patients with malignant glioma is always quite poor, and this poor prognosis is probably due to our incomplete understanding of the molecular mechanisms underlying malignant glioma. It is known that myocyte enhancer factor-2D (MEF2D) plays an oncogenic role in hepatocellular carcinoma and promotes the survival of various types of cells. However, little is known about the expression profile and function of MEF2D in malignant glioma. In this study, we investigated the function and expression of MEF2D in malignant glioma. We found that in malignant glioma, there is an aberrantly high expression of MEF2D, which leads to poor prognosis of malignant glioma. The downregulation of MEF2D suppresses the proliferation of malignant glioma cell lines by inducing delay of S and G2/M phases of cell cycle and promoting apoptosis. Furthermore, the overexpression of MEF2D in astrocytes accelerates cell proliferation by regulating cell cycle progression. Furthermore, a mouse malignant glioma model demonstrated that MEF2D deficiency blocks malignant glioma formation in vivo. We conclude that MEF2D may act as a potential oncogene in malignant glioma and thus serve as a candidate target for malignant glioma therapy.

Keywords

MEF2D Malignant glioma Cell cycle Apoptosis 

Supplementary material

13277_2015_3791_MOESM1_ESM.docx (20 kb)
Table S1 The relationship between MEF2D expression profiling and WHO grading, IDH 1/2 mutation, histological classification. The relationship between MEF2D expression profiling and WHO Grading based on the data from qRT-PCR and histological staining. The correlation was determined with Spearman’s rho. The relationship between MEF2D expression profiling and IDH 1/2 mutation based on the data from qRT-PCR and PCR-HRM. The correlation was determined with Spearman’s rho. The relationship between patients’ glioma histological classification and MEF2D status based on the data from histological staining and qRT-PCR. The correlation was determined with Spearman’s rho. (DOCX 20 kb)

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Copyright information

© International Society of Oncology and BioMarkers (ISOBM) 2015

Authors and Affiliations

  • Youguang Zhao
    • 1
    • 2
  • Ying Li
    • 3
  • Yuan Ma
    • 4
  • Songtao Wang
    • 5
  • Jingmin Cheng
    • 4
  • Tao Yang
    • 4
  • Zhiyong Sun
    • 4
  • Yongqin Kuang
    • 4
  • Haidong Huang
    • 4
  • Kexia Fan
    • 4
  • Jianwen Gu
    • 4
    • 6
  1. 1.Department of PostgraduateThird Military Medical UniversityChongqingPeople’s Republic of China
  2. 2.Department of UrologyChengdu Military General HospitalChengduPeople’s Republic of China
  3. 3.Department of CardiologyChengdu Military General HospitalChengduPeople’s Republic of China
  4. 4.Department of NeurosurgeryChengdu Military General HospitalChengduPeople’s Republic of China
  5. 5.Section of Scientific Research and TrainingChengdu Military General HospitalChengduPeople’s Republic of China
  6. 6.The 306th Hospital of PLABeijingPeople’s Republic of China

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